BACKGROUND: Sodium hypochlorite (NaOCl), the primary component of household bleach, has been shown to alter the purified mouse allergen Mus m 1, such that antibody recognition, or immunogenicity, is lost. Results of initial experiments suggest that antibody recognition is lost at lower concentrations of NaOCl than those required to fragment Mus m 1. OBJECTIVE: We sought to determine whether NaOCl had similar effects on recombinant (r)Fel d 1 and whether the loss of antibody recognition correlated with the loss of biologic activity, as measured with a basophil histamine release assay. METHODS: Recombinant Fel d 1 was treated with increasing amounts of NaOCl, and the product of the reaction was analyzed by using SDS-PAGE, Western blotting, and ELISA. The biologic activity of NaOCl-treated rFel d 1 was analyzed with a basophil histamine release assay. RESULTS: The protein fragmented at an NaOCl/rFel d 1 molar ratio of 7000, whereas cat-specific IgG recognition was lost at a lower molar ratio of 560. Basophil histamine release assays were performed to determine the effect of NaOCl on the biologic activity of rFel d 1. An NaOCl/protein molar ratio of 70 caused a significant reduction in histamine release from basophils of subjects with cat allergy. A molar ratio of 140 further inhibited histamine release by rFel d 1, suggesting a dose-response relationship between NaOCl and loss of biologic activity. CONCLUSIONS: NaOCl modifies rFel d 1, resulting in loss of immunogenicity and attenuation of biologic activity, as measured by its ability to stimulate basophil histamine release.
BACKGROUND:Sodium hypochlorite (NaOCl), the primary component of household bleach, has been shown to alter the purified mouse allergen Mus m 1, such that antibody recognition, or immunogenicity, is lost. Results of initial experiments suggest that antibody recognition is lost at lower concentrations of NaOCl than those required to fragment Mus m 1. OBJECTIVE: We sought to determine whether NaOCl had similar effects on recombinant (r)Fel d 1 and whether the loss of antibody recognition correlated with the loss of biologic activity, as measured with a basophil histamine release assay. METHODS: Recombinant Fel d 1 was treated with increasing amounts of NaOCl, and the product of the reaction was analyzed by using SDS-PAGE, Western blotting, and ELISA. The biologic activity of NaOCl-treated rFel d 1 was analyzed with a basophil histamine release assay. RESULTS: The protein fragmented at an NaOCl/rFel d 1 molar ratio of 7000, whereas cat-specific IgG recognition was lost at a lower molar ratio of 560. Basophil histamine release assays were performed to determine the effect of NaOCl on the biologic activity of rFel d 1. An NaOCl/protein molar ratio of 70 caused a significant reduction in histamine release from basophils of subjects with cat allergy. A molar ratio of 140 further inhibited histamine release by rFel d 1, suggesting a dose-response relationship between NaOCl and loss of biologic activity. CONCLUSIONS:NaOCl modifies rFel d 1, resulting in loss of immunogenicity and attenuation of biologic activity, as measured by its ability to stimulate basophil histamine release.
Authors: Jay Portnoy; Kevin Kennedy; James Sublett; Wanda Phipatanakul; Elizabeth Matsui; Charles Barnes; Carl Grimes; J David Miller; James M Seltzer; P Brock Williams; Jonathan A Bernstein; David I Bernstein; Joann Blessing-Moore; Linda Cox; David A Khan; David M Lang; Richard A Nicklas; John Oppenheimer Journal: Ann Allergy Asthma Immunol Date: 2012-04 Impact factor: 6.347
Authors: Ginger L Chew; Jonathan Wilson; Felicia A Rabito; Faye Grimsley; Shahed Iqbal; Tiina Reponen; Michael L Muilenberg; Peter S Thorne; Dorr G Dearborn; Rebecca L Morley Journal: Environ Health Perspect Date: 2006-12 Impact factor: 9.031