A Schindl1, H Merwald, L Schindl, C Kaun, J Wojta. 1. Department of Dermatology (Division of Special and Environmental Dermatology), University of Medical School, Währinger Gürtel 18-20, A-1090 Vienna, Austria. Andreas.Schindl@akh-wien.ac.at
Abstract
BACKGROUND: Endothelial cell (EC) proliferation plays a key role in the process of tissue repair. Low-intensity laser irradiation has been demonstrated to accelerate wound healing and to improve microvascularization. OBJECTIVES: The present study evaluated a possible stimulatory influence of low-intensity laser irradiation on human umbilical vein endothelial cell (HUVEC) proliferation in a systematic manner. METHODS: Subconfluent cultures of HUVEC were irradiated every other day with a 670-nm diode laser (intensity: 10-65 mW cm(-2), dose: 2-8 J cm(-2)) during a period of 6 days. Cell proliferation was evaluated quantitatively by counting in a haemocytometer. RESULTS: Our data demonstrate a dose-dependent and intensity-dependent stimulatory effect of laser irradiation on HUVEC cell proliferation. Doses of between 2 and 8 J cm(-2) induced statistically significant cell proliferation. Testing different intensities at a constant dose of 8 J cm(-2), 20 and 65 mW cm(-2) induced most pronounced cell proliferation. CONCLUSIONS: Low-intensity laser irradiation influences EC proliferation and might thereby contribute to the increase in angiogenesis and the acceleration of wound healing in vivo.
BACKGROUND: Endothelial cell (EC) proliferation plays a key role in the process of tissue repair. Low-intensity laser irradiation has been demonstrated to accelerate wound healing and to improve microvascularization. OBJECTIVES: The present study evaluated a possible stimulatory influence of low-intensity laser irradiation on human umbilical vein endothelial cell (HUVEC) proliferation in a systematic manner. METHODS: Subconfluent cultures of HUVEC were irradiated every other day with a 670-nm diode laser (intensity: 10-65 mW cm(-2), dose: 2-8 J cm(-2)) during a period of 6 days. Cell proliferation was evaluated quantitatively by counting in a haemocytometer. RESULTS: Our data demonstrate a dose-dependent and intensity-dependent stimulatory effect of laser irradiation on HUVEC cell proliferation. Doses of between 2 and 8 J cm(-2) induced statistically significant cell proliferation. Testing different intensities at a constant dose of 8 J cm(-2), 20 and 65 mW cm(-2) induced most pronounced cell proliferation. CONCLUSIONS: Low-intensity laser irradiation influences EC proliferation and might thereby contribute to the increase in angiogenesis and the acceleration of wound healing in vivo.
Authors: Feng Lin; Steven F Josephs; Doru T Alexandrescu; Famela Ramos; Vladimir Bogin; Vincent Gammill; Constantin A Dasanu; Rosalia De Necochea-Campion; Amit N Patel; Ewa Carrier; David R Koos Journal: J Transl Med Date: 2010-02-16 Impact factor: 5.531
Authors: Lúcio Frigo; Juliana S S Luppi; Giovani M Favero; Durnavei A Maria; Sócrates C Penna; Jan M Bjordal; Rene J Bensadoun; Rodrigo A B Lopes-Martins Journal: BMC Cancer Date: 2009-11-20 Impact factor: 4.430