Solution to the problem of interferences in electrochemical sensors using the fill-and-flow channel biosensor.

A generic fill-and-flow channel biosensor with upstream electrodes to determine the extent of interferences in the sample is described. A pair of upstream electrodes poised at a suitable potential allows both the calculation of the extent of removal of interfering agents and the effect of interfering agents at the detector electrode. A model was developed and tested that predicts the concentrations of all species throughout the channel and, hence, the current at each electrode due to each species. This enables correction of the detector electrode current and a more accurate determination of the analyte concentration. The concept was applied to a biosensor for the determination of glucose in the presence of ascorbic acid, acetamidophenol, and uric acid, as well as glucose in wine samples containing polyphenolic interfering agents.