H L Qin1, T Z Zhao, Y J Shang, Z T Wang. 1. Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China. hlqin@hotmail.com
Abstract
AIM: To assign the 1HNMR finger-print of the root bark of Celastrus angulatus. METHODS: Silica gel column chromatography was used to separate the chemical constituents of CGE A of the root bark of C. angulatus. The characteristic signals of the 1HNMR finger-print were assigned after determining the structures of the compounds isolated from CGE A. RESULTS: 1HNMR finger-prints of the samples of C. angulatus collected from different regions showed highly characteristic features and reproducibility. Three compounds predominant in CGE A were isolated and their structures were determined by spectral analysis as: angulatin A (1), angulatin B (2) and angulatin C (3). CONCLUSION: Compound 3 is a new compound. Compound 2 was isolated from C. angulatus for the first time. The 1HNMR finger-print of CGE A of the root bark of C. angulatus showed mainly the characteristic signals of the above three compounds and might be utilized for the original authentication of this plant.
AIM: To assign the 1HNMR finger-print of the root bark of Celastrus angulatus. METHODS: Silica gel column chromatography was used to separate the chemical constituents of CGE A of the root bark of C. angulatus. The characteristic signals of the 1HNMR finger-print were assigned after determining the structures of the compounds isolated from CGE A. RESULTS: 1HNMR finger-prints of the samples of C. angulatus collected from different regions showed highly characteristic features and reproducibility. Three compounds predominant in CGE A were isolated and their structures were determined by spectral analysis as: angulatin A (1), angulatin B (2) and angulatin C (3). CONCLUSION: Compound 3 is a new compound. Compound 2 was isolated from C. angulatus for the first time. The 1HNMR finger-print of CGE A of the root bark of C. angulatus showed mainly the characteristic signals of the above three compounds and might be utilized for the original authentication of this plant.