Literature DB >> 12582484

Identification of QTLs controlling tissue-culture traits in barley ( Hordeum vulgare L.).

Y. Mano1, T. Komatsuda.   

Abstract

Quantitative trait loci (QTLs) controlling callus growth (CG), subsequent shoot differentiation ratio (SD) and green shoot ratio (GS) in immature embryo culture were identified in barley. A base map was developed from 99 recombinant inbred lines (RILs) of 'Azumamugi' x 'Kanto Nakate Gold'. The tissue-culture traits were evaluated at the F(7) and F(10) generations of the RILs. The RILs showed wide and continuous variations in each of the three tissue-culture traits. Three QTLs for CG, three QTLs for SD and two QTLs for GS were detected by using composite interval mapping. A QTL for SD on chromosome 3H had a large effect, and 'Kanto Nakate Gold', which has a high differentiation ability, contributed to this QTL. The location of this QTL is identical to, or very close to, the uzu locus. We discuss the relationships between tissue-culture loci in 'Azumamugi' x 'Kanto Nakate Gold' and those in other mapping populations.

Entities:  

Year:  2002        PMID： 12582484     DOI： 10.1007/s00122-002-0992-3

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  19 in total

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Authors:  T Komatsuda; P Maxim; N Senthil; Y Mano
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