| Literature DB >> 12581453 |
C Hattab1, D Blanchard, P Gane, V Verkarre, Y Petit-Leroux, M-J Loirat, J-P Cartron, O Bertrand.
Abstract
Mice immunized with a synthetic peptide located on an intracellular segment of the polytopic Kx protein (37 kDa) from human red blood cells (RBCs) produced a monoclonal antibody called C7B8. As expected, this antibody did not agglutinate common RBCs but reacted with permeabilized cells in flow cytometry. C7B8 recognizes the Kx protein on Western blots. Cross-reactivity of C7B8 with human calpain of human muscle extracts was demonstrated by Western blot analysis. This cross-reactivity precludes the use of C7B8 for Kx tissue distribution studies, but immobilized C7B8 was a convenient tool for purification of the Kell-Kx complex from RBC membrane extract by immunochromatography.Entities:
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Year: 2003 PMID： 12581453 DOI： 10.1046/j.1365-3148.2003.00414.x
Source DB: PubMed Journal: Transfus Med ISSN： 0958-7578 Impact factor: 2.019