Z F Wang1, Z Y Peng, L J Huang, R Lu, G Y Tian. 1. Center of Biological Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Abstract
AIM: To isolate polysaccharide from Spirulina platensis and determine its sugar position, molecular weight and biological activities. METHODS: Sephadex G-75 and CM-Sephadex C-50 were used. The sugar position was analyzed by gas chromatography, the molecular weight was determined by GPC. The homogeneity of this glycoconjugate was determined by HPLC and CE. IR and NMR spectra were used to determine the glycosidic linkage. RESULTS: SPPA-1 was a homogeneous glycoconjugate, its carbohydrate chain was composed of alpha-glucopyranan, carbohydrate content is 91.70%, Nitrogen content was 0.96%, the molecular weight was 69.00 x 10(4), SPPA-1 could eliminate O2-. radicals. CONCLUSION: SPPA-1 was an antioxidative glycoconjugate from Spirulina platensis.
AIM: To isolate polysaccharide from Spirulina platensis and determine its sugar position, molecular weight and biological activities. METHODS:Sephadex G-75 and CM-Sephadex C-50 were used. The sugar position was analyzed by gas chromatography, the molecular weight was determined by GPC. The homogeneity of this glycoconjugate was determined by HPLC and CE. IR and NMR spectra were used to determine the glycosidic linkage. RESULTS: SPPA-1 was a homogeneous glycoconjugate, its carbohydrate chain was composed of alpha-glucopyranan, carbohydrate content is 91.70%, Nitrogen content was 0.96%, the molecular weight was 69.00 x 10(4), SPPA-1 could eliminate O2-. radicals. CONCLUSION: SPPA-1 was an antioxidative glycoconjugate from Spirulina platensis.
Authors: Mohamed F Ismail; Doaa A Ali; Augusta Fernando; Mohamed E Abdraboh; Rajiv L Gaur; Wael M Ibrahim; Madhwa H G Raj; Allal Ouhtit Journal: Int J Biol Sci Date: 2009-06-02 Impact factor: 6.580