X R Li1, D Zhang, Y F Qi. 1. Affiliated Hospital of Shandong TCM University, Jinan 250011.
Abstract
OBJECTIVE: To observe the efficacy of Xiaoliu Pingyi Mixture (XLPY) in inducing the apoptosis of human hepatocellular carcinoma (HCC) cell line H-7402. METHODS: Using serum pharmacologic method, the morphological change of apoptotic cells was observed at different time points by co-culturing with different concentrations of medicated serum containing XLPY with light and fluorescent microscopy, flow cytometry, and immunohistochemical staining methods. Blank serum was taken as control. RESULTS: The different concentration of medicated serum containing XLPY could significantly inhibit the growth of H-7402 cell line in vitro. After treated with medicated serum of XLPY, the cellular morphologic changes were typical apoptosis under light and fluorescent microscopy, the sub-G1 peaks were observed by flow cytometric assays. The apoptosis rate revealed a medicated serum concentration and time dependent manner. The control group 5%, 10% medicated serum induced apoptotic rate were 0.49%, 5.58% and 8.46% by 24 h; while by 48 h, it was 0.83%, 11.58% and 14.25% respectively. bcl-2 gene examination showed that the bcl-2 gene expression was inhibited. CONCLUSION: XLPY could induce apoptosis in carcinoma cells, and play the clinical role of anti-tumor and anti-metastasis.
OBJECTIVE: To observe the efficacy of Xiaoliu Pingyi Mixture (XLPY) in inducing the apoptosis of humanhepatocellular carcinoma (HCC) cell line H-7402. METHODS: Using serum pharmacologic method, the morphological change of apoptotic cells was observed at different time points by co-culturing with different concentrations of medicated serum containing XLPY with light and fluorescent microscopy, flow cytometry, and immunohistochemical staining methods. Blank serum was taken as control. RESULTS: The different concentration of medicated serum containing XLPY could significantly inhibit the growth of H-7402 cell line in vitro. After treated with medicated serum of XLPY, the cellular morphologic changes were typical apoptosis under light and fluorescent microscopy, the sub-G1 peaks were observed by flow cytometric assays. The apoptosis rate revealed a medicated serum concentration and time dependent manner. The control group 5%, 10% medicated serum induced apoptotic rate were 0.49%, 5.58% and 8.46% by 24 h; while by 48 h, it was 0.83%, 11.58% and 14.25% respectively. bcl-2 gene examination showed that the bcl-2 gene expression was inhibited. CONCLUSION: XLPY could induce apoptosis in carcinoma cells, and play the clinical role of anti-tumor and anti-metastasis.