G Y Yang1, P J Wang, X B Jia. 1. Jiangsu Provincial Hospital of Integrated Traditional and Western Medicine, Nanjing 210028.
Abstract
OBJECTIVE: To explore the effect of Bushen Huoxue decoction (BSHX) on female reproduction and elucidate its therapeutic mechanism to infertility. METHODS: The BSHX medicated serum of rabbit, as a supplement, was co-cultured with the sperm and ovum of non-copulated mice, and the 2-cell embryos of copulated female mice separately, to observe the changes of in vitro fertilization rate (IVF) and early embryogenesis rate. RESULTS: By co-cultured with BSHX medicated serum, IVF rate was increased obviously (P < 0.01), and the follow-up early embryogenesis rate at various period was promoted, particularly that of the 4-cell and 8-cell embryos (P < 0.05). No influence on developmental rate of in vitro 4-cell embryos obtained from the in vivo 2-cell embryos. But the development of the 8-cell embryos, morula, blastula and hatching were promoted significantly. CONCLUSION: BSHX could raise the fertilization rate and promote the early embryonic development.
OBJECTIVE: To explore the effect of Bushen Huoxue decoction (BSHX) on female reproduction and elucidate its therapeutic mechanism to infertility. METHODS: The BSHX medicated serum of rabbit, as a supplement, was co-cultured with the sperm and ovum of non-copulated mice, and the 2-cell embryos of copulated female mice separately, to observe the changes of in vitro fertilization rate (IVF) and early embryogenesis rate. RESULTS: By co-cultured with BSHX medicated serum, IVF rate was increased obviously (P < 0.01), and the follow-up early embryogenesis rate at various period was promoted, particularly that of the 4-cell and 8-cell embryos (P < 0.05). No influence on developmental rate of in vitro 4-cell embryos obtained from the in vivo 2-cell embryos. But the development of the 8-cell embryos, morula, blastula and hatching were promoted significantly. CONCLUSION:BSHX could raise the fertilization rate and promote the early embryonic development.
Authors: Huijuan Cao; Mei Han; Ernest H Y Ng; Xiaoke Wu; Andrew Flower; George Lewith; Jian-Ping Liu Journal: PLoS One Date: 2013-12-10 Impact factor: 3.240