Wei Zhang1, A Geller. 1. Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Abstract
OBJECTIVE: This study was conducted to compare the immune response to gene transfer with helper virus or helper virus-free vectors. METHODS: Two vector stocks were delivered by stereotactic injection into the striatum and then the immune response markers including TCR, CD4, CD8, MHC-I and MHC-II were examined. RESULTS: The data showed that the immune response to either vector stock reached maximal levels by 4 to 7 days after gene transfer. However, the maximal levels of all markers to the helper virus-free stock were 3 to 10 folds lower than the markers to the helper virus stock. The immune response to the helper virus-free stock declined rapidly to be close to the levels of PBS control. CONCLUSION: The immune response to the helper virus-free stock is significantly less than the response to the vector stock that contains helper virus.
OBJECTIVE: This study was conducted to compare the immune response to gene transfer with helper virus or helper virus-free vectors. METHODS: Two vector stocks were delivered by stereotactic injection into the striatum and then the immune response markers including TCR, CD4, CD8, MHC-I and MHC-II were examined. RESULTS: The data showed that the immune response to either vector stock reached maximal levels by 4 to 7 days after gene transfer. However, the maximal levels of all markers to the helper virus-free stock were 3 to 10 folds lower than the markers to the helper virus stock. The immune response to the helper virus-free stock declined rapidly to be close to the levels of PBS control. CONCLUSION: The immune response to the helper virus-free stock is significantly less than the response to the vector stock that contains helper virus.
Authors: Mei Sun; Guo-Rong Zhang; Lingxin Kong; Courtney Holmes; Xiaodan Wang; Wei Zhang; David S Goldstein; Alfred I Geller Journal: Hum Gene Ther Date: 2003-03-20 Impact factor: 5.695
Authors: Mei Sun; Lingxin Kong; Xiaodan Wang; Courtney Holmes; Qingsheng Gao; Guo-Rong Zhang; Josef Pfeilschifter; David S Goldstein; Alfred I Geller Journal: Hum Gene Ther Date: 2004-12 Impact factor: 5.695
Authors: Guo-Rong Zhang; Meng Liu; Haiyan Cao; Lingxin Kong; Xiaodan Wang; Jennifer A O'Brien; Shuo-Chieh Wu; Robert G Cook; Alfred I Geller Journal: Hippocampus Date: 2009-05 Impact factor: 3.899