Literature DB >> 12571035

Small, acid-soluble proteins as biomarkers in mass spectrometry analysis of Bacillus spores.

Yetrib Hathout1, Barbara Setlow, Rosa-Martha Cabrera-Martinez, Catherine Fenselau, Peter Setlow.   

Abstract

The use of 1 N HCl for extraction of small, acid-soluble proteins (SASP) from different Bacillus spore species was examined. The extracts were analyzed by high-performance liquid chromatography and matrix-assisted laser desorption mass spectrometry and were found to be both qualitatively and quantitatively superior to extraction by acetonitrile-5% trifluoroacetic acid (70:30, vol/vol). Both major and minor alpha/beta- and gamma-type SASP were characterized by their molecular masses or tryptic peptide maps and by searches of both protein and unannotated genome databases. For all but 1 pair (B. cereus T and B. thuringiensis subsp. Kurstaki) among the 11 variants studied the suites of SASP masses are distinctive, consistent with the use of these proteins as potential biomarkers for spore identification by mass spectrometry.

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Year:  2003        PMID： 12571035      PMCID： PMC143666          DOI： 10.1128/AEM.69.2.1100-1107.2003

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  22 in total

1.  Identification of Bacillus spores by matrix-assisted laser desorption ionization-mass spectrometry.

Authors:  Y Hathout; P A Demirev; Y P Ho; J L Bundy; V Ryzhov; L Sapp; J Stutler; J Jackman; C Fenselau
Journal:  Appl Environ Microbiol       Date:  1999-10       Impact factor: 4.792

2.  Cloning and nucleotide sequencing of genes for small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus, and "Thermoactinomyces thalpophilus".

Authors:  C A Loshon; E R Fliss; B Setlow; H F Foerster; P Setlow
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

3.  Essential role of small, acid-soluble spore proteins in resistance of Bacillus subtilis spores to UV light.

Authors:  J M Mason; P Setlow
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

4.  Properties of spores of Bacillus subtilis strains which lack the major small, acid-soluble protein.

Authors:  R H Hackett; P Setlow
Journal:  J Bacteriol       Date:  1988-03       Impact factor: 3.490

5.  Different small, acid-soluble proteins of the alpha/beta type have interchangeable roles in the heat and UV radiation resistance of Bacillus subtilis spores.

Authors:  J M Mason; P Setlow
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

6.  Cloning and nucleotide sequencing of genes for a second type of small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus, and "Thermoactinomyces thalpophilus".

Authors:  D X Sun; P Setlow
Journal:  J Bacteriol       Date:  1987-07       Impact factor: 3.490

7.  Expression of a Bacillus megaterium sporulation-specific gene during sporulation of Bacillus subtilis.

Authors:  S Goldrick; P Setlow
Journal:  J Bacteriol       Date:  1983-09       Impact factor: 3.490

8.  Effects of mutant small, acid-soluble spore proteins from Bacillus subtilis on DNA in vivo and in vitro.

Authors:  F Tovar-Rojo; P Setlow
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

Review 9.  Mechanisms for the prevention of damage to DNA in spores of Bacillus species.

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Review 10.  Antiseptics and disinfectants: activity, action, and resistance.

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Journal:  Clin Microbiol Rev       Date:  1999-01       Impact factor: 26.132
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Authors:  Erica L McJimpsey; William M Jackson; Carlito B Lebrilla; Herbert Tobias; Michael J Bogan; Eric E Gard; Matthias Frank; Paul T Steele
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3.  Microwave supported hydrolysis prepares Bacillus spores for proteomic analysis.

Authors:  Dapeng Chen; Wayne A Bryden; Catherine Fenselau
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4.  Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial.

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Journal:  J Clin Microbiol       Date:  2015-06-10       Impact factor: 5.948

5.  Targeted proteomics approach to species-level identification of Bacillus thuringiensis spores by AP-MALDI-MS.

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6.  Identifying experimental surrogates for Bacillus anthracis spores: a review.

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7.  Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks.

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8.  Species differentiation of a diverse suite of Bacillus spores by mass spectrometry-based protein profiling.

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Journal:  Appl Environ Microbiol       Date:  2004-01       Impact factor: 4.792

9.  Rapid identification of Bacillus anthracis spores in suspicious powder samples by using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).

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10.  Identification and validation of specific markers of Bacillus anthracis spores by proteomics and genomics approaches.

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