Literature DB >> 1257050

Studies on the mechanism of DNA cleavage by ethidium.

I S Deniss, A R Morgan.   

Abstract

Ethidium causes the cleavage of DNA via a light and oxygen dependent process. Using covalently closed circular DNA as a substrate, the saturation kinetics and the dependence on superhelical density of the cleavage indicate that intercalated ethidium is mainly responsible for nicking DNA. Superoxide dismutase has little effect on the reaction and catalase none. Lowering the pH inhibited the reaction. The reaction mechanism and its use in determining superhelical densities of covalently closed circular DNA's are discussed.

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Year:  1976        PMID： 1257050      PMCID： PMC342903          DOI： 10.1093/nar/3.2.315

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

1.  The sense of naturally occurring superhelices and the unwinding angle of intercalated ethidium.

Authors:  D E Pulleyblank; A R Morgan
Journal:  J Mol Biol       Date:  1975-01-05       Impact factor: 5.469

2.  On the structure of the folded chromosome of Escherichia coli.

Authors:  A Worcel; E Burgi
Journal:  J Mol Biol       Date:  1972-11-14       Impact factor: 5.469

3.  The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.

Authors:  J C Wang
Journal:  J Mol Biol       Date:  1974-11-15       Impact factor: 5.469

4.  The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

Authors:  W Bauer; J Vinograd
Journal:  J Mol Biol       Date:  1968-04-14       Impact factor: 5.469

5.  Native and denatured DNA, cross-linked and palindromic DNA and circular covalently-closed DNA analysed by a sensitive fluorometric procedure.

Authors:  A R Morgan; D E Pulleyblank
Journal:  Biochem Biophys Res Commun       Date:  1974-11-27       Impact factor: 3.575

6.  Production of superoxide ions by photosensitization of dyes.

Authors:  C Balny; P Douzou
Journal:  Biochem Biophys Res Commun       Date:  1974-01-23       Impact factor: 3.575

7.  Electron microscopic study of the ethidium bromide-DNA complex.

Authors:  D Freifelder
Journal:  J Mol Biol       Date:  1971-09-14       Impact factor: 5.469

8.  The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen.

Authors:  J M McCord; I Fridovich
Journal:  J Biol Chem       Date:  1969-11-25       Impact factor: 5.157

9.  F 1 sex factor of Escherichia coli. Size and purification in the form of a strand-specific relaxation complex of supercoiled deoxyribonucleic acid and protein.

Authors:  B C Kline; D R Helinski
Journal:  Biochemistry       Date:  1971-12-21       Impact factor: 3.162

10.  Free radicals and inflammation: protection of synovial fluid by superoxide dismutase.

Authors:  J M McCord
Journal:  Science       Date:  1974-08-09       Impact factor: 47.728
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  10 in total

Review 1.  Mechanisms of ATP dependent chromatin remodeling.

Authors:  Vamsi K Gangaraju; Blaine Bartholomew
Journal:  Mutat Res       Date:  2007-01-21       Impact factor: 2.433

2.  Ethidium analogues with improved resolution in the dye-buoyant density procedure.

Authors:  B Hudson; J H Dawson; R Desiderio; C W Mosher
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

3.  Strand breakage in solutions of DNA and ethidium bromide exposed to visible light.

Authors:  P A Martens; D A Clayton
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

Review 4.  Review: ethidium fluorescence assay. Part II. Enzymatic studies and DNA-protein interactions.

Authors:  A R Morgan; D H Evans; J S Lee; D E Pulleyblank
Journal:  Nucleic Acids Res       Date:  1979-10-10       Impact factor: 16.971

5.  Regulation of bacterial DNA supercoiling: plasmid linking numbers vary with growth temperature.

Authors:  E Goldstein; K Drlica
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

6.  A study of the interactions of some polypyridylruthenium (II) complexes with DNA using fluorescence spectroscopy, topoisomerisation and thermal denaturation.

Authors:  J M Kelly; A B Tossi; D J McConnell; C OhUigin
Journal:  Nucleic Acids Res       Date:  1985-09-11       Impact factor: 16.971

Review 7.  Review: ethidium fluorescence assays. Part 1. Physicochemical studies.

Authors:  A R Morgan; J S Lee; D E Pulleyblank; N L Murray; D H Evans
Journal:  Nucleic Acids Res       Date:  1979-10-10       Impact factor: 16.971

8.  Large laterally ordered nanochannel arrays from DNA combing and imprinting.

Authors:  Jingjiao Guan; Pouyan E Boukany; Orin Hemminger; Nan-Rong Chiou; Weibin Zha; Megan Cavanaugh; L James Lee
Journal:  Adv Mater       Date:  2010-09-22       Impact factor: 30.849

9.  DNA sequence homology in Rhizobium meliloti plasmids.

Authors:  L Jouanin; P De Lajudie; S Bazetoux; T Huguet
Journal:  Mol Gen Genet       Date:  1981

10.  Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers.

Authors:  Charlie Yu Ming Hsu; Hasan Uludağ
Journal:  BMC Biotechnol       Date:  2008-02-29       Impact factor: 2.563
  10 in total

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