R Li1, L Zhou, Q Liu. 1. Guangzhou University of TCM, 510407.
Abstract
OBJECTIVE: To study the effect of different animal skin on the transdermal speed constant of sinomenine. METHODS: Improved franz-cell was used for the transdermal study, pH6.8 phosphate buffer was used as the receptor and HPLC method was used for the determination of sinomenine through different animal skin. RESULTS: The transdermal speed constant of the skin of hareless mice was much close to which of person. The higuchi equation of sinomenine through skin of hareless mice stored under -20 degrees C for 0d, 3d and 7d were Q = 40.34t1/2 + 196, Q = 38.01t1/2 + 25.3, Q = 41.55t1/2 + 24.7 respectively. CONCLUSION: The results indicate that skin of hareless mice is suitable for the transdermal study of sinomenine, and the effect of storing time (0-7d) under -40 degrees C is not obvious.
OBJECTIVE: To study the effect of different animal skin on the transdermal speed constant of sinomenine. METHODS: Improved franz-cell was used for the transdermal study, pH6.8phosphate buffer was used as the receptor and HPLC method was used for the determination of sinomenine through different animal skin. RESULTS: The transdermal speed constant of the skin of hareless mice was much close to which of person. The higuchi equation of sinomenine through skin of hareless mice stored under -20 degrees C for 0d, 3d and 7d were Q = 40.34t1/2 + 196, Q = 38.01t1/2 + 25.3, Q = 41.55t1/2 + 24.7 respectively. CONCLUSION: The results indicate that skin of hareless mice is suitable for the transdermal study of sinomenine, and the effect of storing time (0-7d) under -40 degrees C is not obvious.