S Tao1, Z Cai, D Yang. 1. Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Abstract
OBJECTIVE: The purpose of this study was to isolate new arbovirus from mosquitoes collected from the northeast part of China during the summer of 1996. METHODS: The cell used for isolation were C6/36 and BHK-21 cells. RESULTS: Two strains (NE97-12 and NE97-31) of coltivirus were isolated among 68 mosquito specimens. The viruses caused cytopathogenic effects on C6/36 cell, but not on BHK-21 cells. They were resistant to both 5-IDU and ether, but sensitive to pH3.0 and temperature at 56 degrees C for 30 minutes. IFA of new isolates with mouse ascitic fluid anti-coltivirus TRT2 strain showed positive reaction. Polyacrylamide gel electrophoresis (PAGE) of two isolates showed 12 segments of double-stranded(ds) RNA and PAGE profiles were 6-5-1, which were different from that of coltivirus TRT2 strain(6-6). There was no cross neutralization between new isolates and TRT2 strain by tissue culture cross-neutralization test. CONCLUSION: The results indicated while new isolates were serologically related to TRT2 strain of coltivirus by IFA, the neutralization test suggests that it represents a distinct serotype. These new isolates isolated firstly from the northeast part of China belong to a new subtype of coltivirus.
OBJECTIVE: The purpose of this study was to isolate new arbovirus from mosquitoes collected from the northeast part of China during the summer of 1996. METHODS: The cell used for isolation were C6/36 and BHK-21 cells. RESULTS: Two strains (NE97-12 and NE97-31) of coltivirus were isolated among 68 mosquito specimens. The viruses caused cytopathogenic effects on C6/36 cell, but not on BHK-21 cells. They were resistant to both 5-IDU and ether, but sensitive to pH3.0 and temperature at 56 degrees C for 30 minutes. IFA of new isolates with mouse ascitic fluid anti-coltivirus TRT2 strain showed positive reaction. Polyacrylamide gel electrophoresis (PAGE) of two isolates showed 12 segments of double-stranded(ds) RNA and PAGE profiles were 6-5-1, which were different from that of coltivirus TRT2 strain(6-6). There was no cross neutralization between new isolates and TRT2 strain by tissue culture cross-neutralization test. CONCLUSION: The results indicated while new isolates were serologically related to TRT2 strain of coltivirus by IFA, the neutralization test suggests that it represents a distinct serotype. These new isolates isolated firstly from the northeast part of China belong to a new subtype of coltivirus.