S P Li1, Y T Chen, C P Jiang, J M Qiu, D G Yu. 1. Institute of Infectious and Parasitic Disease, Chongqing University of Medical Science, Chongqing 400016.
Abstract
OBJECTIVE: To understand the components of the crude antigens of Echinococcus multilocularis protoscoleces. METHODS: Isoelectric focusing was carried out to fractionate the protein components of E. multilocularis protoscoleces. The individual fractions were collected and subjected to SDS-PAGE, the gels were analyzed by a gel analysis system. RESULTS: The pI values of individual fractions varied from 4.0 to 8.5, while the molecule weights of main protein bands were 72/73, 62/63, 42/44, 35/36, 34 and 21/22 kDa. CONCLUSION: An alternative separation method of crude antigens of Echinococcus multilocularis was established.
OBJECTIVE: To understand the components of the crude antigens of Echinococcus multilocularis protoscoleces. METHODS: Isoelectric focusing was carried out to fractionate the protein components of E. multilocularis protoscoleces. The individual fractions were collected and subjected to SDS-PAGE, the gels were analyzed by a gel analysis system. RESULTS: The pI values of individual fractions varied from 4.0 to 8.5, while the molecule weights of main protein bands were 72/73, 62/63, 42/44, 35/36, 34 and 21/22 kDa. CONCLUSION: An alternative separation method of crude antigens of Echinococcus multilocularis was established.