L S Qiu1, Z Feng, Y H Zhang, H Li. 1. Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Abstract
OBJECTIVE: To detect circulating antigen in the patients with schistosomiasis. METHODS: Polyclonal antibody(PcAb)-monoclonal antibody(McAb) sandwich ELISA. RESULTS: Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%-91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections. CONCLUSION: PcAb-McAb sandwich ELISA is a relatively ideal method for circulatling antigen detection.
OBJECTIVE: To detect circulating antigen in the patients with schistosomiasis. METHODS: Polyclonal antibody(PcAb)-monoclonal antibody(McAb) sandwich ELISA. RESULTS: Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%-91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections. CONCLUSION: PcAb-McAb sandwich ELISA is a relatively ideal method for circulatling antigen detection.