B Shi1, R Han. 1. Department of Pharmacology, Institute of Materia Medica, CAMS and PUMC, Beijing 100050.
Abstract
OBJECTIVE: To study the effects of homoharringtonine and isoharringtonine on the induction of apoptosis on human promyelocytic leukemia HL-60 cells. METHODS: By using flow cytometry, DNA gel electrophoresis, microscopy and electron microscopy. RESULTS: It was demonstrated that homoharringtonine and isoharringtonine can induce apoptosis of HL-60 cells significantly and rapidly. The activity of homoharringtonine was higher than that of isoharringtonine, and it was time and concentration dependent. CONCLUSIONS: Both homoharringtonine and isoharringtonine could induce apoptosis of HL-60 cells in terms of morphology, and DNA ladder assay. The activity of homoharringtonine was much higher than that of isoharringtonine.
OBJECTIVE: To study the effects of homoharringtonine and isoharringtonine on the induction of apoptosis on humanpromyelocytic leukemia HL-60 cells. METHODS: By using flow cytometry, DNA gel electrophoresis, microscopy and electron microscopy. RESULTS: It was demonstrated that homoharringtonine and isoharringtonine can induce apoptosis of HL-60 cells significantly and rapidly. The activity of homoharringtonine was higher than that of isoharringtonine, and it was time and concentration dependent. CONCLUSIONS: Both homoharringtonine and isoharringtonine could induce apoptosis of HL-60 cells in terms of morphology, and DNA ladder assay. The activity of homoharringtonine was much higher than that of isoharringtonine.