BACKGROUND: Studies of vaginal physiology and pathophysiology sometime require larger volumes of undiluted cervicovaginal secretions than can be obtained by current methods. A convenient method for self-sampling these secretions outside a clinical setting can facilitate such studies of reproductive health. GOAL: The goal was to develop a vaginal self-sampling method for collecting large volumes of undiluted cervicovaginal secretions. STUDY DESIGN: A menstrual collection device (the Instead cup) was inserted briefly into the vagina to collect secretions that were then retrieved from the cup by centrifugation in a 50-ml conical tube. RESULTS: All 16 women asked to perform this procedure found it feasible and acceptable. Among 27 samples, an average of 0.5 g of secretions (range, 0.1-1.5 g) was collected. CONCLUSIONS: This is a rapid and convenient self-sampling method for obtaining relatively large volumes of undiluted cervicovaginal secretions. It should prove suitable for a wide range of assays, including those involving sexually transmitted diseases, microbicides, vaginal physiology, immunology, and pathophysiology.
BACKGROUND: Studies of vaginal physiology and pathophysiology sometime require larger volumes of undiluted cervicovaginal secretions than can be obtained by current methods. A convenient method for self-sampling these secretions outside a clinical setting can facilitate such studies of reproductive health. GOAL: The goal was to develop a vaginal self-sampling method for collecting large volumes of undiluted cervicovaginal secretions. STUDY DESIGN: A menstrual collection device (the Instead cup) was inserted briefly into the vagina to collect secretions that were then retrieved from the cup by centrifugation in a 50-ml conical tube. RESULTS: All 16 women asked to perform this procedure found it feasible and acceptable. Among 27 samples, an average of 0.5 g of secretions (range, 0.1-1.5 g) was collected. CONCLUSIONS: This is a rapid and convenient self-sampling method for obtaining relatively large volumes of undiluted cervicovaginal secretions. It should prove suitable for a wide range of assays, including those involving sexually transmitted diseases, microbicides, vaginal physiology, immunology, and pathophysiology.
Authors: Samuel K Lai; D Elizabeth O'Hanlon; Suzanne Harrold; Stan T Man; Ying-Ying Wang; Richard Cone; Justin Hanes Journal: Proc Natl Acad Sci U S A Date: 2007-01-23 Impact factor: 11.205
Authors: Tao Yu; Ying-Ying Wang; Ming Yang; Craig Schneider; Weixi Zhong; Sarah Pulicare; Woo-Jin Choi; Olcay Mert; Jie Fu; Samuel K Lai; Justin Hanes Journal: Drug Deliv Transl Res Date: 2012-04 Impact factor: 4.617
Authors: Olcay Mert; Samuel K Lai; Laura Ensign; Ming Yang; Ying-Ying Wang; Joseph Wood; Justin Hanes Journal: J Control Release Date: 2011-09-03 Impact factor: 9.776
Authors: Ashish Jachak; Samuel K Lai; Kaoru Hida; Jung Soo Suk; Nina Markovic; Shyam Biswal; Patrick N Breysse; Justin Hanes Journal: Nanotoxicology Date: 2011-07-29 Impact factor: 5.913
Authors: Ming Yang; Samuel K Lai; Tao Yu; Ying-Ying Wang; Christina Happe; Weixi Zhong; Michael Zhang; Abraham Anonuevo; Colleen Fridley; Amy Hung; Jie Fu; Justin Hanes Journal: J Control Release Date: 2014-07-29 Impact factor: 9.776
Authors: Benjamin C Tang; Michelle Dawson; Samuel K Lai; Ying-Ying Wang; Jung Soo Suk; Ming Yang; Pamela Zeitlin; Michael P Boyle; Jie Fu; Justin Hanes Journal: Proc Natl Acad Sci U S A Date: 2009-11-09 Impact factor: 11.205
Authors: Samuel K Lai; Kaoru Hida; Shetha Shukair; Ying-Ying Wang; Anna Figueiredo; Richard Cone; Thomas J Hope; Justin Hanes Journal: J Virol Date: 2009-08-19 Impact factor: 5.103