PURPOSE: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells. DESIGN: Experimental study with a direct live/dead cell staining technique using fluorescent dyes. METHODS: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured. RESULTS: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used. CONCLUSION: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.
PURPOSE: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells. DESIGN: Experimental study with a direct live/dead cell staining technique using fluorescent dyes. METHODS: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured. RESULTS: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used. CONCLUSION: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.
Authors: L Kodjikian; T Richter; M Halberstadt; F Beby; F Flueckiger; M Boehnke; J G Garweg Journal: Graefes Arch Clin Exp Ophthalmol Date: 2005-04-15 Impact factor: 3.117
Authors: S Grisanti; P Szurman; O Tatar; F Gelisken; S Aisenbrey; J Oficjalska-Mlynczak; R Kaczmarek; K U Bartz-Schmidt Journal: Br J Ophthalmol Date: 2004-09 Impact factor: 4.638
Authors: P Stalmans; E J Feron; R Parys-Van Ginderdeuren; A Van Lommel; G R J Melles; M Veckeneer Journal: Br J Ophthalmol Date: 2003-06 Impact factor: 4.638