| Literature DB >> 12560065 |
A Zarghi1, S M Foroutan, A Shafaati, A Khoddam.
Abstract
A rapid, simple and sensitive ion-pair HPLC method has been developed for quantification of metformin in plasma. The assay enables the measurement of metformin for therapeutic drug monitoring with a minimum detectable limit of 20 ng/ml. The method involves simple, one-step extraction procedure and analytical recovery was complete. The separation was performed on an analytical 150 x 4.6 mm i.d. mubondapak C(18) column. The wavelength was set at 235 nm. The mobile phase was 40% acetonitrile, 0.01 M sodium dodecyl sulphate, 0.01 M sodium dihydrogen phosphate, and distilled water to 100%, adjusted to pH 5.1 at a flow rate of 1.5 ml/min. The calibration curve was linear over the concentration range 0.2-2.5 microg/ml. The coefficients of variation for inter-day and intra-day assay were within the range of clinical usefulness.Entities:
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Year: 2003 PMID： 12560065 DOI： 10.1016/s0731-7085(02)00608-8
Source DB: PubMed Journal: J Pharm Biomed Anal ISSN： 0731-7085 Impact factor: 3.935