BACKGROUND & AIMS: Enterotoxigenic Bacteroides fragilis that secrete a zinc-dependent metalloprotease toxin termed the B. fragilis toxin (BFT) have been associated with acute diarrheal disease. BFT rapidly cleaves the extracellular domain of E-cadherin, leading to the complete degradation of the E-cadherin protein. E-cadherin is the primary intercellular adhesion protein of the zonula adherens, and its cytoplasmic domain associates with the nuclear signaling protein beta-catenin. The goal of this study was to examine if BFT triggers beta-catenin nuclear signaling in intestinal epithelial cells. METHODS: Cell biologic and biochemical techniques were combined to address beta-catenin nuclear signaling stimulated by BFT. RESULTS: Loss of membrane-associated E-cadherin after BFT treatment of human colonic epithelial cells (HT29/C1 clone) triggers beta-catenin nuclear localization within 3 hours. Subsequently, c-myc transcription and translation are induced and persistent cellular proliferation ensues, mediated in part by beta-catenin/T-cell factor-dependent transcriptional activation. Cellular proliferation is stimulated by as little as 5 x 10(-10) mol/L BFT. CONCLUSIONS: To our knowledge, BFT is the first bacterial toxin reported to activate T-cell factor-dependent beta-catenin nuclear signaling in intestinal epithelial cells. These results suggest that genetic evolution of this common colonic commensal has rendered an organism with the potential to contribute to oncogenic transformation in the colon.
BACKGROUND & AIMS: Enterotoxigenic Bacteroides fragilis that secrete a zinc-dependent metalloprotease toxin termed the B. fragilis toxin (BFT) have been associated with acute diarrheal disease. BFT rapidly cleaves the extracellular domain of E-cadherin, leading to the complete degradation of the E-cadherin protein. E-cadherin is the primary intercellular adhesion protein of the zonula adherens, and its cytoplasmic domain associates with the nuclear signaling protein beta-catenin. The goal of this study was to examine if BFT triggers beta-catenin nuclear signaling in intestinal epithelial cells. METHODS: Cell biologic and biochemical techniques were combined to address beta-catenin nuclear signaling stimulated by BFT. RESULTS: Loss of membrane-associated E-cadherin after BFT treatment of human colonic epithelial cells (HT29/C1 clone) triggers beta-catenin nuclear localization within 3 hours. Subsequently, c-myc transcription and translation are induced and persistent cellular proliferation ensues, mediated in part by beta-catenin/T-cell factor-dependent transcriptional activation. Cellular proliferation is stimulated by as little as 5 x 10(-10) mol/L BFT. CONCLUSIONS: To our knowledge, BFT is the first bacterial toxin reported to activate T-cell factor-dependent beta-catenin nuclear signaling in intestinal epithelial cells. These results suggest that genetic evolution of this common colonic commensal has rendered an organism with the potential to contribute to oncogenic transformation in the colon.
Authors: Matthew Silbergleit; Adrian A Vasquez; Carol J Miller; Jun Sun; Ikuko Kato Journal: Prog Mol Biol Transl Sci Date: 2020-04-09 Impact factor: 3.622
Authors: Liam Chung; Erik Thiele Orberg; Abby L Geis; June L Chan; Kai Fu; Christina E DeStefano Shields; Christine M Dejea; Payam Fathi; Jie Chen; Benjamin B Finard; Ada J Tam; Florencia McAllister; Hongni Fan; Xinqun Wu; Sudipto Ganguly; Andriana Lebid; Paul Metz; Sara W Van Meerbeke; David L Huso; Elizabeth C Wick; Drew M Pardoll; Fengyi Wan; Shaoguang Wu; Cynthia L Sears; Franck Housseau Journal: Cell Host Microbe Date: 2018-02-01 Impact factor: 21.023
Authors: Ki-Jong Rhee; Shaoguang Wu; Xinqun Wu; David L Huso; Baktiar Karim; Augusto A Franco; Shervin Rabizadeh; Jonathan E Golub; Lauren E Mathews; Jai Shin; R Balfour Sartor; Douglas Golenbock; Abdel R Hamad; Christine M Gan; Franck Housseau; Cynthia L Sears Journal: Infect Immun Date: 2009-02-02 Impact factor: 3.441