[A new osmotolerant and glycerol-highly-producing species--Candida glycerolgenesis Zhuge sp. nov].

The strain WL2002-5 isolated from natural samples and overproduced glycerol from glucose was identified systematically. The WL2002-5 fermented glucose and weakly fermented sucrose; assimilated glucose, sucrose, ethanol, weakly assimilated glycerol and citrate, did not assimilated nitrate, erythritol, arabinitol, mannitol. It grew well on the slopes containing 500 g/L of glucose or 10 mL/L acetate or at the temperature up to 40 degrees C. The minimum water activity for its growth was 0.890. It had a negative reaction with DBB and did vegetative reproduction by budding and easily formed Candida-type pseudohyphae but had no sexual reproduction. The molecular weight of its mitochondrial DNA was 20 kb. We conclude that WL2002-5 is a new species of Candida and nominated it Candida glycerolgenesis Zhuge sp. nov..