Literature DB >> 12554964

The advantages of using a modified microbatch method for rapid screening of protein crystallization conditions.

Allan D'Arcy1, Aengus Mac Sweeney, Martine Stihle, Alexander Haber.   

Abstract

In this study, characterization and optimization of a modified microbatch crystallization technique has been attempted in order to provide a rapid screening method. Using this method for screening has certain advantages over standard vapour-diffusion methods: no sealing of drops is required, no reservoir solutions are needed and the experiments can easily be performed over a range of temperatures.

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Year:  2003        PMID： 12554964     DOI： 10.1107/s0907444902022011

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  23 in total

1.  Laboratory scale structural genomics.

Authors:  Brent W Segelke; Johana Schafer; Matthew A Coleman; Tim P Lekin; Dominique Toppani; Krzysztof J Skowronek; Katherine A Kantardjieff; Bernhard Rupp
Journal:  J Struct Funct Genomics       Date:  2004

2.  Crystallization and preliminary X-ray data analysis of a DJ-1 homologue from Arabidopsis thaliana (AtDJ-1D).

Authors:  Kyung Hye Seo; Ningning Zhuang; Joon-Yung Cha; Daeyoung Son; Kon Ho Lee
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-12-24

3.  Biocrystallography: past, present, future.

Authors:  Richard Giegé; Claude Sauter
Journal:  HFSP J       Date:  2010-04-22

4.  Purification, crystallization and preliminary X-ray diffraction studies of UDP-glucose:tetrahydrobiopterin α-glucosyltransferase (BGluT) from Synechococcus sp. PCC 7942.

Authors:  Asaithambi Killivalavan; Ningning Zhuang; Young Shik Park; Kon Ho Lee
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-01-21       Impact factor: 1.056

5.  Crystallization of recombinant bifunctional nuclease TBN1 from tomato.

Authors:  Tomáš Koval'; Petra Lipovová; Tomáš Podzimek; Jaroslav Matoušek; Jarmila Dušková; Tereza Skálová; Andrea Stěpánková; Jindřich Hašek; Jan Dohnálek
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-12-23

6.  Organophosphorus acid anhydrolase from Alteromonas macleodii: structural study and functional relationship to prolidases.

Authors:  Andrea Štěpánková; Jarmila Dušková; Tereza Skálová; Jindřich Hašek; Tomáš Koval'; Lars H Østergaard; Jan Dohnálek
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2013-03-28

7.  Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases.

Authors:  Nilofer Husain; Karolina L Tkaczuk; Shenoy Rajesh Tulsidas; Katarzyna H Kaminska; Sonja Cubrilo; Gordana Maravić-Vlahovicek; Janusz M Bujnicki; J Sivaraman
Journal:  Nucleic Acids Res       Date:  2010-03-01       Impact factor: 16.971

8.  X-ray structure of glutathione S-transferase from Schistosoma japonicum in a new crystal form reveals flexibility of the substrate-binding site.

Authors:  Arne Christian Rufer; Lars Thiebach; Kristin Baer; Helmut W Klein; Michael Hennig
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-02-24

9.  Protein Crystallization in an Actuated Microfluidic Nanowell Device.

Authors:  Bahige G Abdallah; Shatabdi Roy-Chowdhury; Raimund Fromme; Petra Fromme; Alexandra Ros
Journal:  Cryst Growth Des       Date:  2016-02-25       Impact factor: 4.076

10.  Crystal structure of thioflavin T bound to the peripheral site of Torpedo californica acetylcholinesterase reveals how thioflavin T acts as a sensitive fluorescent reporter of ligand binding to the acylation site.

Authors:  Michal Harel; Leilani K Sonoda; Israel Silman; Joel L Sussman; Terrone L Rosenberry
Journal:  J Am Chem Soc       Date:  2008-05-31       Impact factor: 15.419
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