R E Scott1, S Gao. 1. Department of Pathology, University of Tennessee Health Science Center, 3 North Dunlap Street, Memphis, TN 38163, USA. rescott@utmem.edu
Abstract
BACKGROUND: Cell cycle progression from G1 through S to mitosis can be influenced by microtubule-dependent mechanisms that involve AP1 factors and c-Jun N-terminal kinase (JNK) activity. UV irradiation-induced apoptosis also involves AP1 factors and JNK activity. The current studies evaluated the outcome of P2P-R deficiency on these mechanisms because P2P-R expression is repressed in association with a decrease in AP1 inducibility and cell cycle progression during differentiation, and P2P-R overexpression promotes apoptosis. MATERIALS AND METHODS: The ability of the microtubule disruption drug nocodazole to induce mitotic arrest and the ability of UV irradiation to induce apoptosis was evaluated in native versus cells made P2P-R deficient by P2P-R antisense treatment. RESULTS: P2P-R deficiency restricts cell cycle progression from G1 through S to mitosis in a microtubule-dependent manner and P2P-R deficiency represses UV irradiation-induced apoptosis. CONCLUSION: P2P-R may influence AP1 and/or JNK signaling pathways.
BACKGROUND: Cell cycle progression from G1 through S to mitosis can be influenced by microtubule-dependent mechanisms that involve AP1 factors and c-Jun N-terminal kinase (JNK) activity. UV irradiation-induced apoptosis also involves AP1 factors and JNK activity. The current studies evaluated the outcome of P2P-R deficiency on these mechanisms because P2P-R expression is repressed in association with a decrease in AP1 inducibility and cell cycle progression during differentiation, and P2P-R overexpression promotes apoptosis. MATERIALS AND METHODS: The ability of the microtubule disruption drug nocodazole to induce mitotic arrest and the ability of UV irradiation to induce apoptosis was evaluated in native versus cells made P2P-R deficient by P2P-R antisense treatment. RESULTS:P2P-R deficiency restricts cell cycle progression from G1 through S to mitosis in a microtubule-dependent manner and P2P-R deficiency represses UV irradiation-induced apoptosis. CONCLUSION:P2P-R may influence AP1 and/or JNK signaling pathways.
Authors: Mautin A Kappo; Eiso Ab; Faqeer Hassem; R Andrew Atkinson; Andrew Faro; Victor Muleya; Takalani Mulaudzi; John O Poole; Jean M McKenzie; Moredreck Chibi; Joanna C Moolman-Smook; D Jasper G Rees; David J R Pugh Journal: J Biol Chem Date: 2011-11-29 Impact factor: 5.157