Literature DB >> 12547032

High-throughput protein expression for the post-genomic era.

Stephen P Chambers1.   

Abstract

In the past, protein expression has been perceived as the principle bottleneck in protein characterization and structure determination. The challenge now is to rapidly express large numbers of genes in the search for new drug targets and therapeutic proteins encoded by the human genome. In this competitive environment, several high-throughput expression strategies for protein production are being used to industrialize the process of protein expression.

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Year:  2002        PMID: 12547032     DOI: 10.1016/s1359-6446(02)02361-9

Source DB:  PubMed          Journal:  Drug Discov Today        ISSN: 1359-6446            Impact factor:   7.851


  6 in total

1.  Genome-scale expression of proteins from Bacillus subtilis.

Authors:  S Moy; L Dieckman; M Schiffer; N Maltsev; G-X Yu; F R Collart
Journal:  J Struct Funct Genomics       Date:  2004

2.  New insights into the structure-function relationships of Rho-associated kinase: a thermodynamic and hydrodynamic study of the dimer-to-monomer transition and its kinetic implications.

Authors:  John D Doran; Xun Liu; Paul Taslimi; Ahmad Saadat; Ted Fox
Journal:  Biochem J       Date:  2004-12-01       Impact factor: 3.857

3.  Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyrase.

Authors:  Steven Bellon; Jonathan D Parsons; Yunyi Wei; Koto Hayakawa; Lora L Swenson; Paul S Charifson; Judith A Lippke; Robert Aldape; Christian H Gross
Journal:  Antimicrob Agents Chemother       Date:  2004-05       Impact factor: 5.191

4.  Identifying protein construct variants with increased crystallization propensity--a case study.

Authors:  Guido A Malawski; Roman C Hillig; Felipe Monteclaro; Uwe Eberspaecher; Arndt A P Schmitz; Kerstin Crusius; Martina Huber; Ursula Egner; Peter Donner; Beate Müller-Tiemann
Journal:  Protein Sci       Date:  2006-12       Impact factor: 6.725

5.  Investigation of protein refolding using a fractional factorial screen: a study of reagent effects and interactions.

Authors:  Melissa Swope Willis; James K Hogan; Prakash Prabhakar; Xun Liu; Kuenhi Tsai; Yunyi Wei; Ted Fox
Journal:  Protein Sci       Date:  2005-06-03       Impact factor: 6.725

6.  High-throughput expression of C. elegans proteins.

Authors:  Chi-Hao Luan; Shihong Qiu; James B Finley; Mike Carson; Rita J Gray; Wenying Huang; David Johnson; Jun Tsao; Jérôme Reboul; Philippe Vaglio; David E Hill; Marc Vidal; Lawrence J Delucas; Ming Luo
Journal:  Genome Res       Date:  2004-10       Impact factor: 9.043

  6 in total

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