| Literature DB >> 12546135 |
Karamarie Fecho1, Donald T Lysle.
Abstract
The present study determined the pharmacological effects of acute morphine treatment on the granulocyte phase of the peritoneal inflammatory response to thioglycollate (TG) in rats. Dual-color flow cytometry using anti-CD11b/c-PE mAb in combination with HIS48-FITC mAb allowed for the determination of morphine's effects on 4 inflammatory cell subsets: CD11b/c(+)HIS48med granulocytes; CD11b/c(hi)HIS48neg/lo activated macrophages; CD11b/c(-)HIS48(-) lymphocytes; and CD11b/c(+)HIS48hi cells (a monocyte/macrophage and granulocyte subset). Morphine produced a dose-dependent increase in a select subset of inflammatory peritoneal cells, the CD11b/c(+)HIS48med granulocytes. The effect of morphine was time-dependent, with significant effects first apparent at 4 hr after TG, but the administration of morphine 1 hr before or simultaneously with TG produced a similar increase in CD11b/c(+)HIS48med granulocytes. Naltrexone completely antagonized the morphine-induced increase in CD11b/c(+)HIS48med granulocytes. Collectively, these studies show that a single administration of morphine produces a time-dependent, dose-dependent, opioid receptor-mediated enhancement in the peritoneal granulocyte response to TG.Entities:
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Year: 2002 PMID： 12546135 DOI： 10.1023/a:1021408500630
Source DB: PubMed Journal: Inflammation ISSN： 0360-3997 Impact factor: 4.092