OBJECTIVE: To examine the influence of genetic variations in heat shock proteins on trauma outcome. DESIGN: Prospective, noninterventional, single-center study. SETTING: Level I trauma center. SUBJECTS: Eighty consecutive severe multiple trauma patients. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Plasma concentrations of interleukin-6 and tumor necrosis factor-alpha were measured over a 5-day course by chemiluminescence-immunoassay. The genotypes of the polymorphisms HSPA1B (HSP70-2) G1538A and HSPA1L (HSP70-Hom) C2437T were determined by polymerase chain reaction and restriction cleavage with PstlI or NcoI, respectively. Allele frequency of the HSPA1B 1538 G allele was 0.569, and that of the HSPA1L 2437 T allele was 0.821. Interleukin-6 concentrations rapidly increased and dropped to almost normal after 5 days, whereas tumor necrosis factor-alpha concentrations increased until day 5. Patients carrying the genotypes HSPA1B AG or HSPA1L CT had significantly higher plasma concentrations of tumor necrosis factor-alpha and interleukin-6 compared with those with genotype GG or TT. Presence of the HSPA1L genotype CT also was a significant risk factor to develop liver failure (odds ratio, 4.6; 95% confidence interval, 1.5-14.1) and to acquire at least one complication severe enough to score three points according to the Denver multiple organ failure score (odds ratio, 3.0; 95% confidence interval, 1.1-9.2). CONCLUSION: The data indicate that genetic variations of the heat shock proteins HSPA1B and HSPA1L may contribute to clinical outcome after severe injury.
OBJECTIVE: To examine the influence of genetic variations in heat shock proteins on trauma outcome. DESIGN: Prospective, noninterventional, single-center study. SETTING: Level I trauma center. SUBJECTS: Eighty consecutive severe multiple traumapatients. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Plasma concentrations of interleukin-6 and tumor necrosis factor-alpha were measured over a 5-day course by chemiluminescence-immunoassay. The genotypes of the polymorphisms HSPA1B (HSP70-2) G1538A and HSPA1L (HSP70-Hom) C2437T were determined by polymerase chain reaction and restriction cleavage with PstlI or NcoI, respectively. Allele frequency of the HSPA1B 1538 G allele was 0.569, and that of the HSPA1L 2437 T allele was 0.821. Interleukin-6 concentrations rapidly increased and dropped to almost normal after 5 days, whereas tumor necrosis factor-alpha concentrations increased until day 5. Patients carrying the genotypes HSPA1B AG or HSPA1L CT had significantly higher plasma concentrations of tumor necrosis factor-alpha and interleukin-6 compared with those with genotype GG or TT. Presence of the HSPA1L genotype CT also was a significant risk factor to develop liver failure (odds ratio, 4.6; 95% confidence interval, 1.5-14.1) and to acquire at least one complication severe enough to score three points according to the Denver multiple organ failure score (odds ratio, 3.0; 95% confidence interval, 1.1-9.2). CONCLUSION: The data indicate that genetic variations of the heat shock proteins HSPA1B and HSPA1L may contribute to clinical outcome after severe injury.
Authors: B D Freeman; C R Kennedy; H L Frankel; B Clarridge; D Bolcic-Jankovic; E Iverson; E Shehane; A Celious; B A Zehnbauer; T G Buchman Journal: Pharmacogenomics J Date: 2009-12-08 Impact factor: 3.550
Authors: Prakasha Kempaiah; Karol Dokladny; Zachary Karim; Evans Raballah; John M Ong'echa; Pope L Moseley; Douglas J Perkins Journal: Mol Med Date: 2016-08-30 Impact factor: 6.354
Authors: Petrus R de Jong; Alvin W L Schadenberg; Nicolaas J G Jansen; Berent J Prakken Journal: Cell Stress Chaperones Date: 2008-07-31 Impact factor: 3.667
Authors: Carmen L Contreras-Sesvold; Nyamkhishig Sambuughin; Andrei Blokhin; Patricia A Deuster Journal: Cell Stress Chaperones Date: 2009-08-06 Impact factor: 3.667
Authors: Annalise M Martin; David Nolan; Silvana Gaudieri; Coral Ann Almeida; Richard Nolan; Ian James; Filipa Carvalho; Elizabeth Phillips; Frank T Christiansen; Anthony W Purcell; James McCluskey; Simon Mallal Journal: Proc Natl Acad Sci U S A Date: 2004-03-15 Impact factor: 11.205