BACKGROUND: Diagnostic problems in identifying congenital infection cases in infancy have thus far impaired the assessment of the role of congenital cytomegalovirus (CMV) infection in the etiology of sensorineural hearing loss (SNHL). OBJECTIVE: To estimate the impact of congenital infection in children with SNHL by detection of CMV DNA in stored samples of neonatal dried blood (dried blood spots test). METHODS: The Guthrie cards of 130 children with hearing loss >40 dB hearing loss were retrieved from the regional screening center. CMV DNA was extracted by thermal shock and amplified by PCR. RESULTS: The percentage of SNHL cases attributable to congenital CMV infection was 10% (9 of 87) in infants whose SNHL had been diagnosed in their first 2 months of life and 34.2% (13 of 38) in children with deafness of unidentified cause that was diagnosed in early childhood. In the latter group 42.7% (12 of 28) of the children with a hearing loss of >70 dB were CMV-positive. CONCLUSIONS: The results suggest that congenital CMV infection has a more relevant role in the etiology of SNHL than previously reported. The data obtained in both groups suggest that 20 to 30% of all deafness cases are caused by CMV. The percent of congenital CMV cases alone appears to account for all the cases previously attributed to all congenital infections. More than 40% of deafness cases with an unknown cause, needing rehabilitation, are caused by congenital CMV.
BACKGROUND: Diagnostic problems in identifying congenital infection cases in infancy have thus far impaired the assessment of the role of congenital cytomegalovirus (CMV) infection in the etiology of sensorineural hearing loss (SNHL). OBJECTIVE: To estimate the impact of congenital infection in children with SNHL by detection of CMV DNA in stored samples of neonatal dried blood (dried blood spots test). METHODS: The Guthrie cards of 130 children with hearing loss >40 dB hearing loss were retrieved from the regional screening center. CMV DNA was extracted by thermal shock and amplified by PCR. RESULTS: The percentage of SNHL cases attributable to congenital CMV infection was 10% (9 of 87) in infants whose SNHL had been diagnosed in their first 2 months of life and 34.2% (13 of 38) in children with deafness of unidentified cause that was diagnosed in early childhood. In the latter group 42.7% (12 of 28) of the children with a hearing loss of >70 dB were CMV-positive. CONCLUSIONS: The results suggest that congenital CMV infection has a more relevant role in the etiology of SNHL than previously reported. The data obtained in both groups suggest that 20 to 30% of all deafness cases are caused by CMV. The percent of congenital CMV cases alone appears to account for all the cases previously attributed to all congenital infections. More than 40% of deafness cases with an unknown cause, needing rehabilitation, are caused by congenital CMV.
Authors: Karen B Fowler; Faye P McCollister; Diane L Sabo; Angela G Shoup; Kris E Owen; Julie L Woodruff; Edith Cox; Lisa S Mohamed; Daniel I Choo; Suresh B Boppana Journal: Pediatrics Date: 2017-01-03 Impact factor: 7.124
Authors: Lori Scanga; Shu Chaing; Cynthia Powell; Arthur S Aylsworth; Lizzie J Harrell; Nancy G Henshaw; Chris J Civalier; Leigh B Thorne; Karen Weck; Jessica Booker; Margaret L Gulley Journal: J Mol Diagn Date: 2006-05 Impact factor: 5.568
Authors: Ivan Gentile; Emanuela Zappulo; Maria Pia Riccio; Sandro Binda; Laura Bubba; Laura Pellegrinelli; Domenico Scognamiglio; Francesca Operto; Lucia Margari; Guglielmo Borgia; Carmela Bravaccio Journal: In Vivo Date: 2017 May-Jun Impact factor: 2.155
Authors: Jannick Verbeeck; Erwin Van Kerschaver; Elke Wollants; Kurt Beuselinck; Luc Stappaerts; Marc Van Ranst Journal: J Clin Microbiol Date: 2008-09-03 Impact factor: 5.948