| Literature DB >> 12542636 |
T Mizutani1, M Kobayashi, Y Eshita, O Inanami, T Yamamori, A Goto, Y Ako, H Miyoshi, H Miyamoto, H Kariwa, M Kuwabara, I Takashima.
Abstract
When Western blot analysis of heat-killed bacteria- and lipopolysaccharide (LPS)-treated Aedes albopictus mosquito cell line C6/36 was performed using antiphospholyrated c-Jun amino-terminal kinase (JNK) antibodies, approximately 46 kDa protein was clearly detected with a peak around 30 min. After the C6/36 cells were incubated at 45 degrees C in order to induce apoptosis, the 46 kDa protein continued to be detected for at least 3 h. The internalization of fluorescein-labelled bacteria was inhibited by a JNK-specific inhibitor SP600125, suggesting that phagocytosis involves the JNK signalling pathway in mosquito cells. Based on these results, we found one candidate for the nucleotide sequence of JNK (Ae-JNK) from the C6/36 cells. This study is the first report regarding the mitogen-activated protein kinase (MAPK) of mosquito.Entities:
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Year: 2003 PMID： 12542636 DOI： 10.1046/j.1365-2583.2003.00387.x
Source DB: PubMed Journal: Insect Mol Biol ISSN： 0962-1075 Impact factor: 3.585