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Literature DB >> 12542397

Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro.

Carylyn J Marek¹, Gary A Cameron, Lucy J Elrick, Gabrielle M Hawksworth, Matthew C Wright.

Abstract

The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.

Entities: Disease

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Year: 2003 PMID： 12542397 PMCID： PMC1223247 DOI： 10.1042/BJ20021545

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

33 in total

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Journal: Biochem J Date: 1993-02-01 Impact factor: 3.857

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Journal: Biochem Biophys Res Commun Date: 1993-12-15 Impact factor: 3.575

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Journal: Arch Biochem Biophys Date: 1988-06 Impact factor: 4.013

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Journal: Cancer Lett Date: 1979-08 Impact factor: 8.679

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Authors: M C Wright; A J Paine
Journal: Biochem Biophys Res Commun Date: 1994-06-15 Impact factor: 3.575

16 in total

1. Serine/threonine protein kinase SGK1 in glucocorticoid-dependent transdifferentiation of pancreatic acinar cells to hepatocytes.

Authors: Karen Wallace; Quan Long; Emma A Fairhall; Keith A Charlton; Matthew C Wright
Journal: J Cell Sci Date: 2011-01-11 Impact factor: 5.285

2. Pregnenolone-16alpha-carbonitrile inhibits rodent liver fibrogenesis via PXR (pregnane X receptor)-dependent and PXR-independent mechanisms.

Authors: Carylyn J Marek; Steven J Tucker; Dimitrios K Konstantinou; Lucy J Elrick; Dee Haefner; Charalambos Sigalas; Graeme I Murray; Bryan Goodwin; Matthew C Wright
Journal: Biochem J Date: 2005-05-01 Impact factor: 3.857

3. Hepatocyte-like cells transdifferentiated from a pancreatic origin can support replication of hepatitis B virus.

Authors: Robert Yung-Liang Wang; Chia-Ning Shen; Min-Hui Lin; David Tosh; Chiaho Shih
Journal: J Virol Date: 2005-10 Impact factor: 5.103

4. Disrupted pancreatic exocrine differentiation and malabsorption in response to chronic elevated systemic glucocorticoid.

Authors: Karen Wallace; Paul A Flecknell; Alastair D Burt; Matthew C Wright
Journal: Am J Pathol Date: 2010-07-22 Impact factor: 4.307