[Determination of furazolidone, carbenoxolone sodium and berberine hydrochloride in wei kang tablets by reversed-phase high performance liquid chromatography(RP-HPLC)].

An HPLC method with gradient elution was developed to separate and determine furazolidone, carbenoxolone sodium and berberine hydrochloride in Wei Kang Tablets on Nova-Pak C18 column (150 mm x 3.9 mm i.d., 4 microns), using acetonitrile-phosphate buffer solution (pH 7.0) as mobile phase and 3,5-dinitrobenzoic acid as the internal standard. Detection was performed with UV detector at 254 nm. The calibration curves were linear within the ranges of 141.2 mg/L -1,270.8 mg/L for furazolidone(r = 0.9997), 100.6 mg/L -905.4 mg/L for carbenoxolone sodium(r = 0.9995) and 99.2 mg/L -892.8 mg/L for berberine hydrochloride(r = 0.9991). The recoveries and RSDs were 99.5% and 1.38% for furazolidone, 100.3% and 1.73% for carbenoxolone sodium, 97.3% and 1.97% for berberine hydrochloride respectively. The results show that this method is simple, rapid, specific, accurate and reproducible.