OBJECTIVE: The aim of this study was to examine interleukin-1 beta (IL-1 beta) mRNA and tumor necrosis factor alpha (TNF-alpha) mRNA expression in gingival tissues of patients with adult periodontitis. METHODS: A total of 19 patients, including 10 patients with adult periodontitis and 9 healthy individuals as the control, were evaluated for were selected for evaluating IL-1 beta mRNA and TNF-alpha mRNA expression, using reverse transcription polymerase chain reaction (RT-PCR) technique. RESULTS: The relative amount of IL-1 beta mRNA expression of gingival samples from patients with adult periodontitis (0.819 +/- 0.045) (P < 0.01) was much higher than that from the normal control (0.306 +/- 0.087) (P < 0.01). The relative amount of TNF-alpha mRNA expression, gingival samples from AP patients (0.696 +/- 0.098) was also much higher than that from the normal control (0) (P < 0.01). CONCLUSION: As the inflammatory and bone absorption mediators, IL-1 beta and TNF-alpha might play important roles in the pathogenesis and development of adult periodontitis.
OBJECTIVE: The aim of this study was to examine interleukin-1 beta (IL-1 beta) mRNA and tumor necrosis factor alpha (TNF-alpha) mRNA expression in gingival tissues of patients with adult periodontitis. METHODS: A total of 19 patients, including 10 patients with adult periodontitis and 9 healthy individuals as the control, were evaluated for were selected for evaluating IL-1 beta mRNA and TNF-alpha mRNA expression, using reverse transcription polymerase chain reaction (RT-PCR) technique. RESULTS: The relative amount of IL-1 beta mRNA expression of gingival samples from patients with adult periodontitis (0.819 +/- 0.045) (P < 0.01) was much higher than that from the normal control (0.306 +/- 0.087) (P < 0.01). The relative amount of TNF-alpha mRNA expression, gingival samples from AP patients (0.696 +/- 0.098) was also much higher than that from the normal control (0) (P < 0.01). CONCLUSION: As the inflammatory and bone absorption mediators, IL-1 beta and TNF-alpha might play important roles in the pathogenesis and development of adult periodontitis.