BACKGROUND: Vascular restenosis attributable to intimal thickening remains a major problem after percutaneous transluminal coronary angioplasty (PTCA). METHODS AND RESULTS: Through differential-display analysis, we have identified a novel gene whose expression was increased after catheter injury of rabbit aorta. The gene that is expressed predominantly in vascular smooth muscle cells encodes a novel protein with 7 transmembrane domains, and we termed it ITR (intimal thickness-related receptor). The ITR sequence contains a motif common to the Rhodopsin-like GPCR (G-protein-coupled receptor) superfamily. In vivo analyses of this gene revealed that expression of ITR protein increased with intimal thickening induced by cuff placement around murine femoral artery. Furthermore, ITR-knockout mice were found to be resistant to this experimental intimal thickening. CONCLUSIONS: ITR thus seems to be a novel receptor that may play a role in vascular remodeling and that may represent a good target for development of drugs in the prevention of vascular restenosis.
BACKGROUND:Vascular restenosis attributable to intimal thickening remains a major problem after percutaneous transluminal coronary angioplasty (PTCA). METHODS AND RESULTS: Through differential-display analysis, we have identified a novel gene whose expression was increased after catheter injury of rabbit aorta. The gene that is expressed predominantly in vascular smooth muscle cells encodes a novel protein with 7 transmembrane domains, and we termed it ITR (intimal thickness-related receptor). The ITR sequence contains a motif common to the Rhodopsin-like GPCR (G-protein-coupled receptor) superfamily. In vivo analyses of this gene revealed that expression of ITR protein increased with intimal thickening induced by cuff placement around murine femoral artery. Furthermore, ITR-knockout mice were found to be resistant to this experimental intimal thickening. CONCLUSIONS:ITR thus seems to be a novel receptor that may play a role in vascular remodeling and that may represent a good target for development of drugs in the prevention of vascular restenosis.
Authors: Amy S Etheridge; Paul J Gallins; Dereje Jima; K Alaine Broadaway; Mark J Ratain; Erin Schuetz; Eric Schadt; Adrian Schroder; Cliona Molony; Yihui Zhou; Karen L Mohlke; Fred A Wright; Federico Innocenti Journal: Clin Pharmacol Ther Date: 2020-01-30 Impact factor: 6.875
Authors: Dong Hoon Kang; Mina Choi; Soyoung Chang; Min Young Lee; Doo Jae Lee; Kyungsun Choi; Junseong Park; Eun Chun Han; Daehee Hwang; Kihwan Kwon; Hanjoong Jo; Chulhee Choi; Sang Won Kang Journal: PLoS One Date: 2015-08-25 Impact factor: 3.240