BACKGROUND: The mechanisms responsible for the association between advanced age and atherosclerotic diseases are not clear. Because atherosclerosis develops in response to local endothelial injuries, we investigated the effect of aging on vascular healing and reendothelialization. METHODS AND RESULTS: Endothelium denudation was performed by balloon angioplasty of the iliac arteries in young and old New Zealand White rabbits. Planimetric analysis after Evans Blue staining at 28 days after injury showed a significant decrease in reendothelialization in old versus young animals, which was associated with an important increase in neointimal formation in old rabbits. Vascular endothelial growth factor (VEGF) was rapidly induced after balloon injury. However, arterial VEGF expression was significantly reduced in old versus young animals. To confirm the role of VEGF in the age-dependent impairment of reendothelialization, an adenoviral vector encoding for VEGF(165) (adeno-VEGF) was locally delivered at the time of iliac artery angioplasty. Compared with animals treated with the control vector (adeno-betaGal), reendothelialization was significantly improved and neointimal formation reduced in old rabbits treated with adeno-VEGF. CONCLUSIONS: These results document for the first time an age-dependent impairment of reendothelialization after arterial injury. Our study indicates that VEGF supplementation may represent a useful strategy to accelerate reendothelialization and improve vascular healing in the context of aging.
BACKGROUND: The mechanisms responsible for the association between advanced age and atherosclerotic diseases are not clear. Because atherosclerosis develops in response to local endothelial injuries, we investigated the effect of aging on vascular healing and reendothelialization. METHODS AND RESULTS: Endothelium denudation was performed by balloon angioplasty of the iliac arteries in young and old New Zealand White rabbits. Planimetric analysis after Evans Blue staining at 28 days after injury showed a significant decrease in reendothelialization in old versus young animals, which was associated with an important increase in neointimal formation in old rabbits. Vascular endothelial growth factor (VEGF) was rapidly induced after balloon injury. However, arterial VEGF expression was significantly reduced in old versus young animals. To confirm the role of VEGF in the age-dependent impairment of reendothelialization, an adenoviral vector encoding for VEGF(165) (adeno-VEGF) was locally delivered at the time of iliac artery angioplasty. Compared with animals treated with the control vector (adeno-betaGal), reendothelialization was significantly improved and neointimal formation reduced in old rabbits treated with adeno-VEGF. CONCLUSIONS: These results document for the first time an age-dependent impairment of reendothelialization after arterial injury. Our study indicates that VEGF supplementation may represent a useful strategy to accelerate reendothelialization and improve vascular healing in the context of aging.
Authors: Vikram Sood; Cathy Luke; Erin Miller; Mayo Mitsuya; Gilbert R Upchurch; Thomas W Wakefield; Dan D Myers; Peter K Henke Journal: Ann Vasc Surg Date: 2010-02 Impact factor: 1.466
Authors: Anna Luisa Kühn; Peter Kan; Nils Henninger; Visish Srinivasan; Katyucia de Macedo Rodrigues; Ajay K Wakhloo; Matthew J Gounis; Ajit S Puri Journal: J Clin Neurosci Date: 2019-05-06 Impact factor: 1.961
Authors: Daria K Moaveni; Erin M Lynch; Cathy Luke; Vikram Sood; Gilbert R Upchurch; Thomas W Wakefield; Peter K Henke Journal: J Vasc Surg Date: 2008-03 Impact factor: 4.268
Authors: Laura Lecce; Yuen Ting Lam; Laura A Lindsay; Sui Ching Yuen; Philippa J L Simpson; David J Handelsman; Martin K C Ng Journal: Mol Endocrinol Date: 2014-07-24
Authors: Fatma Vatansever; Natalia C Rodrigues; Livia L Assis; Sabrina S Peviani; Joao L Durigan; Fernando M A Moreira; Michael R Hamblin; Nivaldo A Parizotto Journal: Photonics Lasers Med Date: 2012-10-01
Authors: Augustina Frimpong; Jones Amponsah; Dorothy Agyemang; Abigail Sena Adjokatseh; Sophia Eyiah-Ampah; Nana Aba Ennuson; Dorotheah Obiri; Linda Eva Amoah; Kwadwo Asamoah Kusi Journal: Open Forum Infect Dis Date: 2021-06-04 Impact factor: 3.835