OBJECTIVE: To investigate the 192 Gln-Arg polymorphism of paraoxonase (PON) gene and its relationship with serum lipids levels in patients with coronary heart disease in Chinese population. METHODS: The genotype and allele frequency of paraoxonase gene 192 Gln-Arg polymorphism was assayed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Serum lipids were measured by enzymatic kits in 118 CHD patients and 128 healthy subjects whose fasting serum TG levels were < 1.82 mmol/L and TC levels < 6.2 mmol/L from a population of Chinese Han nationality in Chengdu area. RESULTS: Both in CHD group and control group, the QR genotype of PON gene was the major one, and its frequencies were 0.578 and 0.525 respectively. No differences were found in PON gene Gln-Arg polymophism in the CHD group when compared with the control group. The frequency of R allele of PON gene in Chinese was significant higher than that in European and American Caucasians (0.51 vs. 0.26-0.31, P < 0.01) and Indian (0.51 vs. 0.17, P < 0.01), and significantly lower than that in Japanese (0.51 vs. 0.65, P < 0.01). CONCLUSION: These results suggested that the Gln-Arg192 polymorphism of the PON1 gene was not associated with coronary heart disease in Chinese population.
OBJECTIVE: To investigate the 192 Gln-Arg polymorphism of paraoxonase (PON) gene and its relationship with serum lipids levels in patients with coronary heart disease in Chinese population. METHODS: The genotype and allele frequency of paraoxonase gene 192 Gln-Arg polymorphism was assayed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Serum lipids were measured by enzymatic kits in 118 CHD patients and 128 healthy subjects whose fasting serum TG levels were < 1.82 mmol/L and TC levels < 6.2 mmol/L from a population of Chinese Han nationality in Chengdu area. RESULTS: Both in CHD group and control group, the QR genotype of PON gene was the major one, and its frequencies were 0.578 and 0.525 respectively. No differences were found in PON gene Gln-Arg polymophism in the CHD group when compared with the control group. The frequency of R allele of PON gene in Chinese was significant higher than that in European and American Caucasians (0.51 vs. 0.26-0.31, P < 0.01) and Indian (0.51 vs. 0.17, P < 0.01), and significantly lower than that in Japanese (0.51 vs. 0.65, P < 0.01). CONCLUSION: These results suggested that the Gln-Arg192 polymorphism of the PON1 gene was not associated with coronary heart disease in Chinese population.
Authors: Debbie A Lawlor; Ian N M Day; Tom R Gaunt; Lesley J Hinks; Patricia J Briggs; Matthew Kiessling; Nick Timpson; George Davey Smith; Shah Ebrahim Journal: BMC Genet Date: 2004-06-23 Impact factor: 2.797