S Qu1, Z Wang, Q Fang, G Chen. 1. Department of Nephrology, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Abstract
OBJECTIVE: To investigate the effects of Angiotensin(Ang) II on the proliferation and expression of Interleukin (IL)-6 in human kidney fibroblasts(KFB). METHODS: The human KFB were cultured and identified, the proliferation of KFB was measured by MTT, and the level of IL-6 was measured by radioimmunoassay(RIA). RESULTS: Angiotensin II could stimulate the proliferation of KFB and enhance the expression of IL-6 in protein level on KFB. CONCLUSION: Angiotensin II can stimulate KFB's proliferation and enhance the expression of IL-6 of KFB. These findings suggest that Ang II might play a part in the mechanisms for modulating tubulointerstitial changes and induceing renal fibrosis.
OBJECTIVE: To investigate the effects of Angiotensin(Ang) II on the proliferation and expression of Interleukin (IL)-6 in human kidney fibroblasts(KFB). METHODS: The human KFB were cultured and identified, the proliferation of KFB was measured by MTT, and the level of IL-6 was measured by radioimmunoassay(RIA). RESULTS:Angiotensin II could stimulate the proliferation of KFB and enhance the expression of IL-6 in protein level on KFB. CONCLUSION:Angiotensin II can stimulate KFB's proliferation and enhance the expression of IL-6 of KFB. These findings suggest that Ang II might play a part in the mechanisms for modulating tubulointerstitial changes and induceing renal fibrosis.