Che Chen1, Fu-Kun Liu, Xiao-Ping Qi, Jie-Shou Li. 1. Department of General Surgery, Jinling Hospital, 305 Zhongshandong Road, Nanjing 210002, China. drchenche@sohu.com
Abstract
AIM: To study the influence of chemotherapy on proliferation activation of tumor cell by observing the change of chemiluminescence (CL) and cell cycle in various tumor cell lines after mitomycin C treated. METHODS: BGC823 and LoVo cell lines were all cultured in RPMI-1640, and then were adjusted to a concentration of 1X10(5) cells/ml in fresh media and incubated for 24 h. Mitomycin C (100 ng.ml(-1)) was added to each bottle. All indeses were examined after 24 h. No Mitomycin C was added in control group. Each group contained 8 samples. Flow cytometric analysis and luminol-dependent CL were used to investigate the effect of mitomycin C on two gastrointestinal carcinoma cell lines. RESULTS: BGC823 and LoVo cell lines incubated with MMC for 24 h. We discovered that the emergence of peak of CL stimulated by PHA was postponed significantly (BGC823: 12.63+/-3.21 vs 4.50+/-1.04, LoVo: 13.25+/-2.96 vs 5.12+/-1.36, P<0.01) and the peak intension of CL was reduced significantly (BGC823: 120.25+/-16.61 vs 248.38+/-29.17, LoVo: 98.13+/-10.49 vs 267.50+/-18.56, P<0.01). The PI of cell lines was decreased significantly (BGC823: 51.87+/-4.82 vs 25.44+/-2.26, LoVo: 47.11+/-1.04 vs 24.23+/-0.37, P<0.01) and the apoptotic fractions changed by contraries (BGC823: 26.25+/-5.29 vs 9.83+/-2.51, LoVo: 33.50+/-3.68 vs 9.63+/-1.44, P<0.01). CONCLUSION: CL can be used to measure activation of tumor cells. We discovered that the ground CL intensions of two cell lines were not high but increased rapidly after stimulation of PHA. The CL peak ranged from 4-5 minutes, and then decreased gradually. The results were not reported before. CL of tumor cell has close correlativity with the dynamics of cell cycle and can reflect the feature of oxidation metabolism and proliferation activation of tumor cell. So it can be used to observe the influence of chemotherapy drug on metabolism and proliferation activation of tumor cell and screen out chemotherapy drugs to which tumor cells are sensitive.
AIM: To study the influence of chemotherapy on proliferation activation of tumor cell by observing the change of chemiluminescence (CL) and cell cycle in various tumor cell lines after mitomycin C treated. METHODS: BGC823 and LoVo cell lines were all cultured in RPMI-1640, and then were adjusted to a concentration of 1X10(5) cells/ml in fresh media and incubated for 24 h. Mitomycin C (100 ng.ml(-1)) was added to each bottle. All indeses were examined after 24 h. No Mitomycin C was added in control group. Each group contained 8 samples. Flow cytometric analysis and luminol-dependent CL were used to investigate the effect of mitomycin C on two gastrointestinal carcinoma cell lines. RESULTS: BGC823 and LoVo cell lines incubated with MMC for 24 h. We discovered that the emergence of peak of CL stimulated by PHA was postponed significantly (BGC823: 12.63+/-3.21 vs 4.50+/-1.04, LoVo: 13.25+/-2.96 vs 5.12+/-1.36, P<0.01) and the peak intension of CL was reduced significantly (BGC823: 120.25+/-16.61 vs 248.38+/-29.17, LoVo: 98.13+/-10.49 vs 267.50+/-18.56, P<0.01). The PI of cell lines was decreased significantly (BGC823: 51.87+/-4.82 vs 25.44+/-2.26, LoVo: 47.11+/-1.04 vs 24.23+/-0.37, P<0.01) and the apoptotic fractions changed by contraries (BGC823: 26.25+/-5.29 vs 9.83+/-2.51, LoVo: 33.50+/-3.68 vs 9.63+/-1.44, P<0.01). CONCLUSION: CL can be used to measure activation of tumor cells. We discovered that the ground CL intensions of two cell lines were not high but increased rapidly after stimulation of PHA. The CL peak ranged from 4-5 minutes, and then decreased gradually. The results were not reported before. CL of tumor cell has close correlativity with the dynamics of cell cycle and can reflect the feature of oxidation metabolism and proliferation activation of tumor cell. So it can be used to observe the influence of chemotherapy drug on metabolism and proliferation activation of tumor cell and screen out chemotherapy drugs to which tumor cells are sensitive.