Literature DB >> 12531509

A rapid method for RNA preparation from Gram-positive bacteria.

Eun Taex Oh1, Jae-Seong So.   

Abstract

We have developed a rapid method for preparation of RNA from various Gram-positive bacteria. Unlike some methods, this method does not require lysozyme and proteinase K. Instead, we used glass beads to break cells more efficiently. Using this method, we successfully isolated the total RNA from various Gram-positive bacteria. This method is rapid, simple, and more economic when compared to the previously reported ones. Prepared RNA can be used for the transcriptional analysis of various Gram-positive bacteria.

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Year:  2003        PMID： 12531509     DOI： 10.1016/s0167-7012(02)00218-x

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  10 in total

1.  Single-pass classification of all noncoding sequences in a bacterial genome using phylogenetic profiles.

Authors:  Antonin Marchais; Magali Naville; Chantal Bohn; Philippe Bouloc; Daniel Gautheret
Journal:  Genome Res       Date:  2009-02-23       Impact factor: 9.043

2.  Small RNA genes expressed from Staphylococcus aureus genomic and pathogenicity islands with specific expression among pathogenic strains.

Authors:  Christophe Pichon; Brice Felden
Journal:  Proc Natl Acad Sci U S A       Date:  2005-09-23       Impact factor: 11.205

3.  5-Hydroxymethylfurfural inhibits Acinetobacter baumannii biofilms: an in vitro study.

Authors:  Karuppiah Vijayakumar; Ramanathan Thirunanasambandham
Journal:  Arch Microbiol       Date:  2020-10-09       Impact factor: 2.552

4.  3, 5-Di-tert-butylphenol combat against Streptococcus mutans by impeding acidogenicity, acidurance and biofilm formation.

Authors:  Karuppiah Vijayakumar; Seralathan MuhilVannan
Journal:  World J Microbiol Biotechnol       Date:  2021-10-20       Impact factor: 3.312

5.  6S RNA-Dependent Susceptibility to RNA Polymerase Inhibitors.

Authors:  Marick Esberard; Marc Hallier; Wenfeng Liu; Claire Morvan; Lionello Bossi; Nara Figueroa-Bossi; Brice Felden; Philippe Bouloc
Journal:  Antimicrob Agents Chemother       Date:  2022-04-07       Impact factor: 5.938

6.  Quebrachitol from Rhizophora mucronata inhibits biofilm formation and virulence production in Staphylococcus epidermidis by impairment of initial attachment and intercellular adhesion.

Authors:  Vijayakumar Karuppiah; Ramanathan Thirunanasambandham
Journal:  Arch Microbiol       Date:  2020-03-07       Impact factor: 2.552

7.  Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism.

Authors:  Chantal Bohn; Candice Rigoulay; Svetlana Chabelskaya; Cynthia M Sharma; Antonin Marchais; Patricia Skorski; Elise Borezée-Durant; Romain Barbet; Eric Jacquet; Annick Jacq; Daniel Gautheret; Brice Felden; Jörg Vogel; Philippe Bouloc
Journal:  Nucleic Acids Res       Date:  2010-05-28       Impact factor: 16.971

8.  No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus.

Authors:  Chantal Bohn; Candice Rigoulay; Philippe Bouloc
Journal:  BMC Microbiol       Date:  2007-02-09       Impact factor: 3.605

9.  Umbelliferone Impedes Biofilm Formation and Virulence of Methicillin-Resistant Staphylococcus epidermidis via Impairment of Initial Attachment and Intercellular Adhesion.

Authors:  Thirukannamangai Krishnan Swetha; Murugesan Pooranachithra; Ganapathy Ashwinkumar Subramenium; Velayutham Divya; Krishnaswamy Balamurugan; Shunmugiah Karutha Pandian
Journal:  Front Cell Infect Microbiol       Date:  2019-10-18       Impact factor: 5.293

10.  Influence of oxygen on NADH recycling and oxidative stress resistance systems in Lactobacillus panis PM1.

Authors:  Tae Sun Kang; Darren R Korber; Takuji Tanaka
Journal:  AMB Express       Date:  2013-01-31       Impact factor: 3.298
  10 in total

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