BACKGROUND/AIMS: The mechanisms of somatostatin-mediated gastroprotection are not fully understood. Aims of this study were to determine in the rat the role of nitric oxide (NO) in somatostatin-induced effects on gastric mucosal protection and blood flow (GMBF) in the absence and in the presence of intraluminal ethanol. METHODS: Ethanol (70% v/v)-induced gastric mucosal injury after orogastric dosing was quantitated at 30 min and GMBF determined in an ex vivo gastric chamber preparation. RESULTS: Somatostatin (4 microg/kg; i.p.) protection against ethanol-induced ulceration was prevented by the NO inhibitor L-NNA and restored by L-arginine, but not D-arginine. Somatostatin (1-8 microg/kg; i.p.) did not effect basal GMBF. The gastroprotective dose of somatostatin (4 microg/kg; i.p.) prevented the decrease in GMBF caused by ethanol. L-NNA reversed this vascular effect of somatostatin. CONCLUSION: Somatostatin-induced gastroprotection and restoration of GMBF during ethanol exposure involve mechanisms which are dependent on NO generation.
BACKGROUND/AIMS: The mechanisms of somatostatin-mediated gastroprotection are not fully understood. Aims of this study were to determine in the rat the role of nitric oxide (NO) in somatostatin-induced effects on gastric mucosal protection and blood flow (GMBF) in the absence and in the presence of intraluminal ethanol. METHODS:Ethanol (70% v/v)-induced gastric mucosal injury after orogastric dosing was quantitated at 30 min and GMBF determined in an ex vivo gastric chamber preparation. RESULTS:Somatostatin (4 microg/kg; i.p.) protection against ethanol-induced ulceration was prevented by the NO inhibitor L-NNA and restored by L-arginine, but not D-arginine. Somatostatin (1-8 microg/kg; i.p.) did not effect basal GMBF. The gastroprotective dose of somatostatin (4 microg/kg; i.p.) prevented the decrease in GMBF caused by ethanol. L-NNA reversed this vascular effect of somatostatin. CONCLUSION:Somatostatin-induced gastroprotection and restoration of GMBF during ethanol exposure involve mechanisms which are dependent on NO generation.
Authors: Larisse Tavares Lucetti; Renan Oliveira Silva; Ana Paula Macedo Santana; Bruno de Melo Tavares; Mariana Lima Vale; Pedro Marcos Gomes Soares; Francisco José Batista de Lima Júnior; Pedro Jorge Caldas Magalhães; Fernando de Queiroz Cunha; Ronaldo de Albuquerque Ribeiro; Jand-Venes Rolim Medeiros; Marcellus Henrique Loiola Ponte Souza Journal: Dig Dis Sci Date: 2016-11-18 Impact factor: 3.199
Authors: A L Rozza; D A S Cesar; L G Pieroni; L L Saldanha; A L Dokkedal; F M De-Faria; A R M Souza-Brito; W Vilegas; R K Takahira; C H Pellizzon Journal: Evid Based Complement Alternat Med Date: 2015-04-12 Impact factor: 2.629