Hu Xia1, Li-min Luo, Jin-xu Wen, Wan-cheng Tong. 1. Department of Respiratory Diseases, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, P. R. China. reptile212@sina.com
Abstract
BACKGROUND & OBJECTIVE: Generally, growth and metastasis of tumor are critically dependent on angiogenesis. Endostatin can specifically inhibits tumor angiogenesis. The current study was designed to evaluate the inhibitory effect of recombinant human endostatin (rhES) secreted by pichia, pastoris, GS115 on the growth and metastasis of mice lung adenocarcinoma LA795 in mice T739. METHODS: To select a strain that could highly express recombinant human endostatin, then induce the clone to express rhES by adding methanol. Purification of rhES was performed with heparin affinity chromatography. LA795 tumor cells were inoculated subcutaneously into the dorsa of T739 mice, and the mice were randomized into two groups. The first group was given rhES(20 mg/kg/d), and the second group was given equal volume of PBS, for 14 consecutive days. The volume of tumors were measured. And the tumor metastasis in the lungs of the mice was observed. RESULTS: The selected clone was induced to secrete enough soluble rhES. The purified protein could strongly inhibit growth and metastasis of mice lung adenocarcinoma LA795 in T739 mice (P < 0.001). CONCLUSION: The rhES secreted by pichia, pastoris, GS115 has good biological activities and greatly inhibit growth and metastasis of mice lung adenocarcinoma LA795 in mice T739.
BACKGROUND & OBJECTIVE: Generally, growth and metastasis of tumor are critically dependent on angiogenesis. Endostatin can specifically inhibits tumor angiogenesis. The current study was designed to evaluate the inhibitory effect of recombinant humanendostatin (rhES) secreted by pichia, pastoris, GS115 on the growth and metastasis of micelung adenocarcinoma LA795 in mice T739. METHODS: To select a strain that could highly express recombinant humanendostatin, then induce the clone to express rhES by adding methanol. Purification of rhES was performed with heparin affinity chromatography. LA795 tumor cells were inoculated subcutaneously into the dorsa of T739 mice, and the mice were randomized into two groups. The first group was given rhES(20 mg/kg/d), and the second group was given equal volume of PBS, for 14 consecutive days. The volume of tumors were measured. And the tumor metastasis in the lungs of the mice was observed. RESULTS: The selected clone was induced to secrete enough soluble rhES. The purified protein could strongly inhibit growth and metastasis of micelung adenocarcinoma LA795 in T739 mice (P < 0.001). CONCLUSION: The rhES secreted by pichia, pastoris, GS115 has good biological activities and greatly inhibit growth and metastasis of micelung adenocarcinoma LA795 in mice T739.