X F Yang1, C Q Song. 1. Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Abstract
OBJECTIVE: To study the metabolism of gentiopicroside by rat intestinal flora in vitro and time profiles of gentiopicroside and its metabolites in rat serum after oral administration of gentiopicroside. METHOD: Using high performance liquid chromatography (HPLC) for determination of gentiopicroside, gentianine and gentianal content of rat intestinal flora culture medium in vitro and rat serum sample in vivo. RESULT: Gentiopicroside was metabolized quickly in vitro by rat intestinal flora, consequently gentianine and gentianal content appeared. Gentianine and gentianal were observed after gentiopicroside oral administration 4 times in rat serum sample, and gentiopicroside content decreased after oral administration 6 times. CONCLUSION: Gentianine and gentianal were the important bioactive metabolites of gentiopicroside in rats.
OBJECTIVE: To study the metabolism of gentiopicroside by rat intestinal flora in vitro and time profiles of gentiopicroside and its metabolites in rat serum after oral administration of gentiopicroside. METHOD: Using high performance liquid chromatography (HPLC) for determination of gentiopicroside, gentianine and gentianal content of rat intestinal flora culture medium in vitro and rat serum sample in vivo. RESULT: Gentiopicroside was metabolized quickly in vitro by rat intestinal flora, consequently gentianine and gentianal content appeared. Gentianine and gentianal were observed after gentiopicroside oral administration 4 times in rat serum sample, and gentiopicroside content decreased after oral administration 6 times. CONCLUSION:Gentianine and gentianal were the important bioactive metabolites of gentiopicroside in rats.
Authors: Chang-Hong Wang; Zheng-Tao Wang; S W Annie Bligh; Kenneth N White; Christopher J Branford White Journal: Eur J Drug Metab Pharmacokinet Date: 2004 Jul-Sep Impact factor: 2.441