OBJECTIVE: To evaluate the expression of granulocyte chemotactic protein-2 (GCP-2) in human endometrial stromal cells. DESIGN: The effects of interleukin (IL)-1alpha, IL-1beta, tumor necrosis factor (TNF)-alpha, TNF-beta, interferon (IFN)-gamma, and lipopolysaccharide (LPS) on the production of GCP-2 by endometrial stromal cells were investigated. SETTING: Research laboratory at a medical university. PATIENT(S): Eight endometrial specimens in the late proliferative phase were used. INTERVENTION(S): Endometrial stromal cells were incubated for 24 hours with IL-1alpha, IL-1beta, TNF-alpha, TNF-beta, IFN-gamma, and LPS.The concentration of GCP-2 in the culture media was measured using an enzyme-linked immunosorbent assay (ELISA). RESULT(S): A small amount of GCP-2 was detected in the culture media of unstimulated endometrial stromal cells. The production of GCP-2 by endometrial stromal cells was stimulated with IL-1alpha, IL-1beta, TNF-alpha, TNF-beta, and LPS in a dose-dependent manner. Interferon-gamma did not affect GCP-2 production by these cells. CONCLUSION(S): These results suggest that GCP-2 is an additional ELR(+)-CXC chemokine expressed in endometrial stromal cells. The modulation of GCP-2 concentrations in the local environment may contribute to the normal and pathological processes of human reproduction by regulating the neutrophil trafficking in the endometrium.
OBJECTIVE: To evaluate the expression of granulocyte chemotactic protein-2 (GCP-2) in human endometrial stromal cells. DESIGN: The effects of interleukin (IL)-1alpha, IL-1beta, tumor necrosis factor (TNF)-alpha, TNF-beta, interferon (IFN)-gamma, and lipopolysaccharide (LPS) on the production of GCP-2 by endometrial stromal cells were investigated. SETTING: Research laboratory at a medical university. PATIENT(S): Eight endometrial specimens in the late proliferative phase were used. INTERVENTION(S): Endometrial stromal cells were incubated for 24 hours with IL-1alpha, IL-1beta, TNF-alpha, TNF-beta, IFN-gamma, and LPS.The concentration of GCP-2 in the culture media was measured using an enzyme-linked immunosorbent assay (ELISA). RESULT(S): A small amount of GCP-2 was detected in the culture media of unstimulated endometrial stromal cells. The production of GCP-2 by endometrial stromal cells was stimulated with IL-1alpha, IL-1beta, TNF-alpha, TNF-beta, and LPS in a dose-dependent manner. Interferon-gamma did not affect GCP-2 production by these cells. CONCLUSION(S): These results suggest that GCP-2 is an additional ELR(+)-CXC chemokine expressed in endometrial stromal cells. The modulation of GCP-2 concentrations in the local environment may contribute to the normal and pathological processes of human reproduction by regulating the neutrophil trafficking in the endometrium.
Authors: Pooja Mittal; Roberto Romero; Juan Pedro Kusanovic; Samuel S Edwin; Francesca Gotsch; Shali Mazaki-Tovi; Jimmy Espinoza; Offer Erez; Chia-Ling Nhan-Chang; Nandor G Than; Edi Vaisbuch; Sonia S Hassan Journal: Am J Reprod Immunol Date: 2008-09 Impact factor: 3.886