Ujjwal Kumar Rout1, Michael P Diamond. 1. C. S. Mott Center for Human Growth and Development, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA. aa4306@wayne.edu
Abstract
OBJECTIVE: Study the expression pattern of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators during uterine serosal healing after lesioning in a rat model. DESIGN: Descriptive study with an adhesion model using reverse transcriptase-polymerase chain reaction (PCR) and immuncytochemical techniques. SETTING: Academic medical center. PATIENT(S): None. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Expression profiles of t-PA and u-PA were examined in the uteroperitoneal adhesion tissues of rat from the time of injury until day 7 after surgery using relative abundance reverse transcriptase-PCR and immunocytochemical techniques. RESULT(S): The t-PA transcript levels dropped in uteroperitoneal tissues by 6 hours after surgery and remained low until day 5. Thereafter, a rapid up-regulation of this transcript was observed at day 7. In contrast, the u-PA transcript levels demonstrated a biphasic profile during the progression of adhesion development with peaks at 2 and 5 days after surgery. Immunocytochemical staining demonstrated expression of these plasminogen activators in the newly formed blood vessels. CONCLUSION(S): Altered levels of t-PA and u-PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development.
OBJECTIVE: Study the expression pattern of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators during uterine serosal healing after lesioning in a rat model. DESIGN: Descriptive study with an adhesion model using reverse transcriptase-polymerase chain reaction (PCR) and immuncytochemical techniques. SETTING: Academic medical center. PATIENT(S): None. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Expression profiles of t-PA and u-PA were examined in the uteroperitoneal adhesion tissues of rat from the time of injury until day 7 after surgery using relative abundance reverse transcriptase-PCR and immunocytochemical techniques. RESULT(S): The t-PA transcript levels dropped in uteroperitoneal tissues by 6 hours after surgery and remained low until day 5. Thereafter, a rapid up-regulation of this transcript was observed at day 7. In contrast, the u-PA transcript levels demonstrated a biphasic profile during the progression of adhesion development with peaks at 2 and 5 days after surgery. Immunocytochemical staining demonstrated expression of these plasminogen activators in the newly formed blood vessels. CONCLUSION(S): Altered levels of t-PA and u-PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development.
Authors: Demetrios Moris; Jeffery Chakedis; Amir A Rahnemai-Azar; Ana Wilson; Mairead Marion Hennessy; Antonios Athanasiou; Eliza W Beal; Chrysoula Argyrou; Evangelos Felekouras; Timothy M Pawlik Journal: J Gastrointest Surg Date: 2017-07-06 Impact factor: 3.452
Authors: Karen L Reed; A Brent Fruin; Adam C Gower; Arthur F Stucchi; Susan E Leeman; James M Becker Journal: Proc Natl Acad Sci U S A Date: 2004-06-08 Impact factor: 11.205
Authors: Jong Ju Jeong; Dong Won Lee; Seung Yong Song; Yerin Park; Ji Hee Kim; Jang Il Kim; Hyung Goo Kim; Ki Taek Nam; Won Jai Lee; Kee-Hyun Nam; Ju Hee Lee Journal: PLoS One Date: 2019-02-22 Impact factor: 3.240