BACKGROUND: The aim of this work was to study the influence of soluble factors produced by native mouse intestinal epithelial cells (IECs) on the proliferative activity of freshly isolated intestinal crypt cells. METHODS: The crypt cells were cultured with either conditioned medium and its ultrafiltrates or recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) in the presence or absence of neutralizing anti-GM-CSF antibodies. GM-CSF in culture medium was identified by the electrochemiluminescence method. RESULTS: It was demonstrated that the IEC conditioned medium contained GM-CSF. This cytokine led to both the upregulation and downregulation of crypt cell proliferative activity, depending on its concentration in the culture medium. The effect of native GM-CSF was reproduced with recombinant mouse GM-CSF: 25 and 5 ng/ml inhibited the proliferative activity, whereas 1 ng/ml led to its significant stimulation. CONCLUSIONS: Freshly isolated murine IECs produce GM-CSF, which plays a critical role in crypt cell proliferative activity in vitro. These results suggest the involvement of this factor in the regulation of the crypt proliferative zone, in an autocrine and/or paracrine manner.
BACKGROUND: The aim of this work was to study the influence of soluble factors produced by native mouse intestinal epithelial cells (IECs) on the proliferative activity of freshly isolated intestinal crypt cells. METHODS: The crypt cells were cultured with either conditioned medium and its ultrafiltrates or recombinant mousegranulocyte-macrophage colony-stimulating factor (GM-CSF) in the presence or absence of neutralizing anti-GM-CSF antibodies. GM-CSF in culture medium was identified by the electrochemiluminescence method. RESULTS: It was demonstrated that the IEC conditioned medium contained GM-CSF. This cytokine led to both the upregulation and downregulation of crypt cell proliferative activity, depending on its concentration in the culture medium. The effect of native GM-CSF was reproduced with recombinant mouseGM-CSF: 25 and 5 ng/ml inhibited the proliferative activity, whereas 1 ng/ml led to its significant stimulation. CONCLUSIONS: Freshly isolated murine IECs produce GM-CSF, which plays a critical role in crypt cell proliferative activity in vitro. These results suggest the involvement of this factor in the regulation of the crypt proliferative zone, in an autocrine and/or paracrine manner.
Authors: Xiaonan Han; Shila Gilbert; Katherine Groschwitz; Simon Hogan; Ingrid Jurickova; Bruce Trapnell; Charles Samson; Jonathan Gully Journal: Gut Date: 2010-06-28 Impact factor: 23.059
Authors: Grace Gathungu; Mi-Ok Kim; John P Ferguson; Yashoda Sharma; Wei Zhang; Sok Meng E Ng; Erin Bonkowski; Kaida Ning; Lisa A Simms; Anthony R Croft; Joanne M Stempak; Nicole Walker; Ning Huang; Yang Xiao; Mark S Silverberg; Bruce Trapnell; Judy H Cho; Graham L Radford-Smith; Lee A Denson Journal: Inflamm Bowel Dis Date: 2013-07 Impact factor: 5.325
Authors: Laia Egea; Christopher S McAllister; Omar Lakhdari; Ivelina Minev; Steve Shenouda; Martin F Kagnoff Journal: J Immunol Date: 2013-01-16 Impact factor: 5.422