Literature DB >> 12517888

Use of real-time PCR to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" DNA.

Séverine Tasker1, Chris R Helps, Michael J Day, Tim J Gruffydd-Jones, Dave A Harbour.   

Abstract

A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.

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Year:  2003        PMID: 12517888      PMCID: PMC149582          DOI: 10.1128/JCM.41.1.439-441.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  9 in total

1.  Use of a polymerase chain reaction assay to detect and differentiate two strains of Haemobartonella felis in naturally infected cats.

Authors:  W A Jensen; M R Lappin; S Kamkar; W J Reagan
Journal:  Am J Vet Res       Date:  2001-04       Impact factor: 1.156

2.  Proposal to transfer some members of the genera Haemobartonella and Eperythrozoon to the genus Mycoplasma with descriptions of 'Candidatus Mycoplasma haemofelis', 'Candidatus Mycoplasma haemomuris', 'Candidatus Mycoplasma haemosuis' and 'Candidatus Mycoplasma wenyonii'.

Authors:  H Neimark; K E Johansson; Y Rikihisa; J G Tully
Journal:  Int J Syst Evol Microbiol       Date:  2001-05       Impact factor: 2.747

3.  'Candidatus Mycoplasma haemominutum', a low-virulence epierythrocytic parasite of cats.

Authors:  J E Foley; N C Pedersen
Journal:  Int J Syst Evol Microbiol       Date:  2001-05       Impact factor: 2.747

4.  Use of a PCR assay to assess the prevalence and risk factors for Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in cats in the United Kingdom.

Authors:  S Tasker; S H Binns; M J Day; T J Gruffydd-Jones; D A Harbour; C R Helps; W A Jensen; C S Olver; M R Lappin
Journal:  Vet Rec       Date:  2003-02-15       Impact factor: 2.695

Review 5.  Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.

Authors:  S A Bustin
Journal:  J Mol Endocrinol       Date:  2000-10       Impact factor: 5.098

6.  Revision of haemotrophic Mycoplasma species names.

Authors:  Harold Neimark; Karl-Erik Johansson; Yasuko Rikihisa; Joseph G Tully
Journal:  Int J Syst Evol Microbiol       Date:  2002-03       Impact factor: 2.747

7.  Inoculation of two genotypes of Hemobartonella felis (California and Ohio variants) to induce infection in cats and the response to treatment with azithromycin.

Authors:  D S Westfall; W A Jensen; W J Reagan; S V Radecki; M R Lappin
Journal:  Am J Vet Res       Date:  2001-05       Impact factor: 1.156

8.  Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats.

Authors:  J E Foley; S Harrus; A Poland; B Chomel; N C Pedersen
Journal:  Am J Vet Res       Date:  1998-12       Impact factor: 1.156

9.  Mycoplasmas (Mollicutes) have a low number of rRNA genes.

Authors:  D Amikam; G Glaser; S Razin
Journal:  J Bacteriol       Date:  1984-04       Impact factor: 3.490

  9 in total
  26 in total

1.  Detection of humoral response using a recombinant heat shock protein 70, DnaK, of Mycoplasma haemofelis in experimentally and naturally hemoplasma-infected cats.

Authors:  Emily N Barker; Chris R Helps; Kate J Heesom; Christopher J Arthur; Iain R Peters; Regina Hofmann-Lehmann; Séverine Tasker
Journal:  Clin Vaccine Immunol       Date:  2010-10-06

2.  Identification, characterization, and application of a recombinant antigen for the serological investigation of feline hemotropic Mycoplasma infections.

Authors:  Godelind A Wolf-Jäckel; Christian Jäckel; Kristina Museux; Katharina Hoelzle; Séverine Tasker; Hans Lutz; Regina Hofmann-Lehmann
Journal:  Clin Vaccine Immunol       Date:  2010-09-28

3.  Demonstration of two distinct hemotropic mycoplasmas in French dogs.

Authors:  Martin J Kenny; Susan E Shaw; Fréderic Beugnet; Séverine Tasker
Journal:  J Clin Microbiol       Date:  2004-11       Impact factor: 5.948

4.  Phylogenetic analysis of "Candidatus Mycoplasma turicensis" isolates from pet cats in the United Kingdom, Australia, and South Africa, with analysis of risk factors for infection.

Authors:  Barbara Willi; Séverine Tasker; Felicitas S Boretti; Marcus G Doherr; Valentino Cattori; Marina L Meli; Remo G Lobetti; Richard Malik; Claudia E Reusch; Hans Lutz; Regina Hofmann-Lehmann
Journal:  J Clin Microbiol       Date:  2006-10-11       Impact factor: 5.948

5.  Real-time PCR investigation of potential vectors, reservoirs, and shedding patterns of feline hemotropic mycoplasmas.

Authors:  Barbara Willi; Felicitas S Boretti; Marina L Meli; Marco V Bernasconi; Simona Casati; Daniel Hegglin; Maria Puorger; Harold Neimark; Valentino Cattori; Nicole Wengi; Claudia E Reusch; Hans Lutz; Regina Hofmann-Lehmann
Journal:  Appl Environ Microbiol       Date:  2007-04-27       Impact factor: 4.792

6.  Identification, molecular characterization, and occurrence of two bovine hemoplasma species in Swiss cattle and development of real-time TaqMan quantitative PCR assays for diagnosis of bovine hemoplasma infections.

Authors:  Marina L Meli; Barbara Willi; Ute M Dreher; Valentino Cattori; Gabriela Knubben-Schweizer; Karl Nuss; Ueli Braun; Hans Lutz; Regina Hofmann-Lehmann
Journal:  J Clin Microbiol       Date:  2010-08-04       Impact factor: 5.948

Review 7.  Haemotropic mycoplasmas: what's their real significance in cats?

Authors:  Séverine Tasker
Journal:  J Feline Med Surg       Date:  2010-05       Impact factor: 2.015

8.  In vivo transmission studies of 'Candidatus Mycoplasma turicensis' in the domestic cat.

Authors:  Kristina Museux; Felicitas S Boretti; Barbara Willi; Barbara Riond; Katharina Hoelzle; Ludwig E Hoelzle; Max M Wittenbrink; Séverine Tasker; Nicole Wengi; Claudia E Reusch; Hans Lutz; Regina Hofmann-Lehmann
Journal:  Vet Res       Date:  2009-05-16       Impact factor: 3.683

9.  Description of outcomes of experimental infection with feline haemoplasmas: copy numbers, haematology, Coombs' testing and blood glucose concentrations.

Authors:  Séverine Tasker; Iain R Peters; Kostas Papasouliotis; Simon M Cue; Barbara Willi; Regina Hofmann-Lehmann; Timothy J Gruffydd-Jones; Toby G Knowles; Michael J Day; Chris R Helps
Journal:  Vet Microbiol       Date:  2009-06-21       Impact factor: 3.293

10.  Distribution of Mycoplasma haemofelis in blood and tissues following experimental infection.

Authors:  Séverine Tasker; Iain R Peters; Michael J Day; Barbara Willi; Regina Hofmann-Lehmann; Timothy J Gruffydd-Jones; Chris R Helps
Journal:  Microb Pathog       Date:  2009-09-23       Impact factor: 3.738

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