OBJECTIVE: The purpose of this study was to investigate whether or not infection of prosthetic vascular graft could be prevented with the placement of antibiotic-loaded porous apatite ceramic on the graft with bacterial contamination. METHODS: Teicoplanin as an antibiotic was loaded into a beta-tricalcium phosphate (TCP) block of porous apatite ceramic. The activity of teicoplanin released from teicoplanin-loaded TCP was examined for 40 days at 2-day intervals. In an in vivo study, the graft was patched in the abdominal aorta of a rabbit. Two different doses of Staphylococcus aureus were applied (n = 13). Teicoplanin-loaded TCP was placed around the graft infected with S aureus (n = 6). The graft, the tissue around it, and the arterial blood were collected and cultivated 4 weeks after operation. RESULTS: The activity of teicoplanin in the replaced saline solution was maintained for at least 40 days. Because graft infection was established in all rabbits with a dose of 2 x 10(7) colony forming units, this dose was used as the dose of S aureus in this study. Fifty-seven percent of the subjects died from the infection. When teicoplanin-loaded TCP was placed around the graft contaminated with S aureus, no rabbits died. Bacterial cultures of the graft and of tissues around it were negative in all rabbits killed 4 weeks after operation. This infection was prevented with teicoplanin-loaded TCP in all rabbits. CONCLUSION: Teicoplanin-loaded TCP may be useful in avoiding Dacron graft infection with S aureus in rabbits.
OBJECTIVE: The purpose of this study was to investigate whether or not infection of prosthetic vascular graft could be prevented with the placement of antibiotic-loaded porous apatite ceramic on the graft with bacterial contamination. METHODS:Teicoplanin as an antibiotic was loaded into a beta-tricalcium phosphate (TCP) block of porous apatite ceramic. The activity of teicoplanin released from teicoplanin-loaded TCP was examined for 40 days at 2-day intervals. In an in vivo study, the graft was patched in the abdominal aorta of a rabbit. Two different doses of Staphylococcus aureus were applied (n = 13). Teicoplanin-loaded TCP was placed around the graft infected with S aureus (n = 6). The graft, the tissue around it, and the arterial blood were collected and cultivated 4 weeks after operation. RESULTS: The activity of teicoplanin in the replaced saline solution was maintained for at least 40 days. Because graft infection was established in all rabbits with a dose of 2 x 10(7) colony forming units, this dose was used as the dose of S aureus in this study. Fifty-seven percent of the subjects died from the infection. When teicoplanin-loaded TCP was placed around the graft contaminated with S aureus, no rabbits died. Bacterial cultures of the graft and of tissues around it were negative in all rabbits killed 4 weeks after operation. This infection was prevented with teicoplanin-loaded TCP in all rabbits. CONCLUSION:Teicoplanin-loaded TCP may be useful in avoiding Dacron graft infection with S aureus in rabbits.
Authors: Aysenur Topsakal; Nazmi Ekren; Osman Kilic; Faik N Oktar; Mahir Mahirogullari; Ozan Ozkan; Hilal Turkoglu Sasmazel; Mustafa Turk; Iuliana M Bogdan; George E Stan; Oguzhan Gunduz Journal: J Mater Sci Mater Med Date: 2020-01-21 Impact factor: 3.896
Authors: N Blanchemain; F Chai; S Haulon; V Krump-Konvalinkova; M Traisnel; M Morcellet; B Martel; C J Kirkpatrick; H F Hildebrand Journal: J Mater Sci Mater Med Date: 2008-02-12 Impact factor: 3.896
Authors: Bulent Mese; Orhan Bozoglan; Serdal Elveren; Erdinc Eroglu; Mustafa Gul; Ahmet Celik; Harun Ciralik; Halil Ibrahim Yildirimdemir; Alptekin Yasim Journal: Med Sci Monit Date: 2015-03-27
Authors: Anna V Piterina; Aidan J Cloonan; Claire L Meaney; Laura M Davis; Anthony Callanan; Michael T Walsh; Tim M McGloughlin Journal: Int J Mol Sci Date: 2009-11-20 Impact factor: 6.208