OBJECTIVE: To investigate whether the 3 most common mutations in the NOD2 gene that confer susceptibility to Crohn's disease (CD) are also associated with ankylosing spondylitis (AS). METHODS: DNA from 112 patients with AS and 168 controls of homogenous Spanish ancestry were studied. The frequencies of the pathogenic alleles of NOD2 (3020insC, 2722G>C, and 2104C>T) were determined by analysis of the melting curves after hybridization with FRET probes on a Light Cycler real-time polymerase chain reaction (PCR) system. Results. NOD2 allelic frequencies in controls (3020insC, 0.009; 2722G>C, 0.009; 2104C>T, 0.042) did not significantly differ from patients with AS (3020insC, 0.009; 2722G>C, 0.004; 2104C>T, 0.031). CONCLUSION: The 3 most common CD NOD2 mutations do not contribute to disease susceptibility to AS, and therefore do not explain the susceptibility locus for AS in chromosome 16q.
OBJECTIVE: To investigate whether the 3 most common mutations in the NOD2 gene that confer susceptibility to Crohn's disease (CD) are also associated with ankylosing spondylitis (AS). METHODS: DNA from 112 patients with AS and 168 controls of homogenous Spanish ancestry were studied. The frequencies of the pathogenic alleles of NOD2 (3020insC, 2722G>C, and 2104C>T) were determined by analysis of the melting curves after hybridization with FRET probes on a Light Cycler real-time polymerase chain reaction (PCR) system. Results. NOD2 allelic frequencies in controls (3020insC, 0.009; 2722G>C, 0.009; 2104C>T, 0.042) did not significantly differ from patients with AS (3020insC, 0.009; 2722G>C, 0.004; 2104C>T, 0.031). CONCLUSION: The 3 most common CDNOD2 mutations do not contribute to disease susceptibility to AS, and therefore do not explain the susceptibility locus for AS in chromosome 16q.
Authors: D Laukens; H Peeters; D Marichal; B Vander Cruyssen; H Mielants; D Elewaut; P Demetter; C Cuvelier; M Van Den Berghe; P Rottiers; E M Veys; E Remaut; L Steidler; F De Keyser; M De Vos Journal: Ann Rheum Dis Date: 2004-11-11 Impact factor: 19.103
Authors: M van der Paardt; J B A Crusius; M H M T de Koning; S A Morré; R J van de Stadt; B A C Dijkmans; A S Peña; I E van der Horst-Bruinsma Journal: Ann Rheum Dis Date: 2005-02 Impact factor: 19.103
Authors: H Peeters; B Vander Cruyssen; D Laukens; P Coucke; D Marichal; M Van Den Berghe; C Cuvelier; E Remaut; H Mielants; F De Keyser; M D Vos Journal: Ann Rheum Dis Date: 2004-09 Impact factor: 19.103