Biosensor-controlled perfusion culture to estimate the viability of cells.

A perfusion cell culture is characterised by the continuous addition of fresh nutrient medium and the withdrawal of an equal volume of used medium, allowing the realisation of cell cultivation conditions that are approximated as closely as possible to the in vivo situation. The combination of a perfusion cell culture with an enzyme glucose biosensor allows the glucose consumption of the cell culture to be monitored continuously. The resulting biosensor-controlled perfusion cell culture is a complex biomonitoring system that is useful for checking the metabolic state of a perfusion cell culture continuously and non-invasively over several days. With this experimental setup, it has been possible to test detrimental external effects on living systems at early stages, in vitro, but under in vivo-like conditions.