Literature DB >> 1250705

An improved method for the separation and quantitation of the modified nucleosides of transfer RNA.

H Rogg, R Brambilla, G Keith, M Staehelin.   

Abstract

A method is described which allows a very efficient determination of the modified nucleosides of tRNA. The technique involves enzymatic degradation of the tRNA to nucleosides at pH 7.6 and their separation by two-dimensional thin-layer chromatography on cellulose-coated aluminum foils. Based on the analysis of two mammalian tRNAs it is shown that the technique is suitable for the determination of chemically unstable nucleosides as well as the ribose-methylated compounds. At least 36 of the 45 known modified nucleosides can be separated and quantitatively determined by the method described. This procedure is especially suitable for the estimation of the nucleoside composition of unlabeled tRNAs as well as for studying the post-transcriptional modifications of tRNA.

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Year:  1976        PMID： 1250705      PMCID： PMC342895          DOI： 10.1093/nar/3.1.285

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  28 in total

1.  SOME NUCLEOTIDE SEQUENCES FROM PARTIALLY PURIFIED TRANSFER RIBONUCLEIC ACIDS.

Authors:  P L BERGQUIST; J F SCOTT
Journal:  Biochim Biophys Acta       Date:  1964-06-22

2.  STRUCTURE OF A RIBONUCLEIC ACID.

Authors:  R W HOLLEY; J APGAR; G A EVERETT; J T MADISON; M MARQUISEE; S H MERRILL; J R PENSWICK; A ZAMIR
Journal:  Science       Date:  1965-03-19       Impact factor: 47.728

3.  Chromatographic studies of nucleic acids. 4. The nucleic acid of the turnip yellow mosaic virus including a note on the nucleic acid of the tomato bushy stunt virus.

Authors:  R MARKHAM; J D SMITH
Journal:  Biochem J       Date:  1951-09       Impact factor: 3.857

4.  The primary structure of two mammalian tRNAs Phe: identity of calf liver and rabbit liver tRNAs Phe.

Authors:  G Keith; J P Ebel; G Dirheimer
Journal:  FEBS Lett       Date:  1974-11-01       Impact factor: 4.124

5.  On the bias of the distribution of the 2'-O-methylribonucleotide constituents of yeast transfer RNA.

Authors:  S Morisawa; E Chargaff
Journal:  Biochim Biophys Acta       Date:  1968-12-17

6.  Ion-exlusion chromatography: analysis and isolation of nucleic acid components, and influence of separation parameters.

Authors:  R P Singhal
Journal:  Arch Biochem Biophys       Date:  1972-10       Impact factor: 4.013

7.  The effect of growth temperatures on the in vivo ribose methylation of Bacillus stearothermophilus transfer RNA.

Authors:  P F Agris; H Koh; D Söll
Journal:  Arch Biochem Biophys       Date:  1973-01       Impact factor: 4.013

8.  Methylated bases of transfer ribonucleic acid from HeLa and L cells.

Authors:  Y Iwanami; G M Brown
Journal:  Arch Biochem Biophys       Date:  1968-03-20       Impact factor: 4.013

9.  Ion-exchange thin-layer chromatography. 18. Detection of purine derivatives in the nanogram range by phosphorescence at 77 degrees K.

Authors:  K Randerath
Journal:  Anal Biochem       Date:  1967-12       Impact factor: 3.365

10.  Analysis of methylated constituents from RNA by thin-layer chromatography.

Authors:  G R Björk; I Svensson
Journal:  Biochim Biophys Acta       Date:  1967-04-18
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  26 in total

1.  Chorismic acid, a key metabolite in modification of tRNA.

Authors:  T G Hagervall; Y H Jönsson; C G Edmonds; J A McCloskey; G R Björk
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

2.  Pleiotropic effects induced by modification deficiency next to the anticodon of tRNA from Salmonella typhimurium LT2.

Authors:  J U Ericson; G R Björk
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

3.  Characterization of a Salmonella typhimurium hisU mutant defective in tRNA precursor processing.

Authors:  L Bossi; M S Ciampi; R Cortese
Journal:  J Bacteriol       Date:  1978-05       Impact factor: 3.490

4.  Escherichia coli mutants with defects in the biosynthesis of 5-methylaminomethyl-2-thio-uridine or 1-methylguanosine in their tRNA.

Authors:  G R Björk; K Kjellin-Stråby
Journal:  J Bacteriol       Date:  1978-02       Impact factor: 3.490

5.  Nucleotide sequence of threonine tRNA from Bacillus subtilis.

Authors:  T Hasegawa; H Ishikura
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971

6.  Nucleotide sequence of a lysine tRNA from Bacillus subtilis.

Authors:  Y Yamada; H Ishikura
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971

7.  A novel method for the determination of post-transcriptional modification in RNA by mass spectrometry.

Authors:  J A Kowalak; S C Pomerantz; P F Crain; J A McCloskey
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971

8.  Novel E. coli mutants deficient in biosynthesis of 5-methylaminomethyl-2-thiouridine.

Authors:  D Elseviers; L A Petrullo; P J Gallagher
Journal:  Nucleic Acids Res       Date:  1984-04-25       Impact factor: 16.971

9.  In vitro methylation of yeast tRNAAsp by rat brain cortical tRNA-(adenine-1) methyltransferase.

Authors:  C E Salas; G Dirheimer
Journal:  Nucleic Acids Res       Date:  1979-03       Impact factor: 16.971

10.  Methylation patterns of mycoplasma transfer and ribosomal ribonucleic acid.

Authors:  C C Hsuchen; D T Dubin
Journal:  J Bacteriol       Date:  1980-12       Impact factor: 3.490
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