PURPOSE: To establish the clinical significance of calcium binding proteins S100A2 and S100A4 during progression of human prostate adenocarcinoma. PATIENTS AND METHODS: Expression pattern of S100A2 and S100A4 was determined in normal human prostate epithelial cells (NHPE); virally transformed prostate epithelial cells (PZ-HPV-7); several human prostate carcinoma cells (22Rv1, DU145, LNCaP, and PC3); tissue samples obtained during transuretheral prostatic resection from patients with benign prostate hyperplasia (BPH), prostatitis, and adenocarcinoma; and paraffin-embedded sections from pair-matched benign and cancer specimens of different tumor grade. RESULTS: High constitutive protein expression of S100A2 was observed in NHPE and PZ-HPV-7 cells, whereas its complete absence was observed in 22Rv1, DU145, LNCaP, and PC3 cells. Tissue samples of BPH and prostatitis exhibited higher mRNA and protein levels of S100A2 than low-grade cancer (Gleason score <or= 6), whereas a complete loss was observed in high-grade cancer specimens (Gleason score > 6). Immunohistochemical analysis further confirmed high levels of S100A2 in benign tissues and a progressive loss with increasing tumor grade. The protein level of S100A4 was significantly higher in all carcinoma cells compared with NHPE and PZ-HPV-7 cells. The mRNA and protein level of S100A4 was significantly higher in high-grade cancer specimens compared with BPH, prostatitis, and low-grade cancer. The high levels of S100A4 observed in cancer tissue correlated with increasing tumor grade. CONCLUSION: Loss of S100A2 and increased expression of S100A4 may be an important event during progression of prostate cancer in humans.
PURPOSE: To establish the clinical significance of calcium binding proteins S100A2 and S100A4 during progression of humanprostate adenocarcinoma. PATIENTS AND METHODS: Expression pattern of S100A2 and S100A4 was determined in normal human prostate epithelial cells (NHPE); virally transformed prostate epithelial cells (PZ-HPV-7); several humanprostate carcinoma cells (22Rv1, DU145, LNCaP, and PC3); tissue samples obtained during transuretheral prostatic resection from patients with benign prostate hyperplasia (BPH), prostatitis, and adenocarcinoma; and paraffin-embedded sections from pair-matched benign and cancer specimens of different tumor grade. RESULTS: High constitutive protein expression of S100A2 was observed in NHPE and PZ-HPV-7 cells, whereas its complete absence was observed in 22Rv1, DU145, LNCaP, and PC3 cells. Tissue samples of BPH and prostatitis exhibited higher mRNA and protein levels of S100A2 than low-grade cancer (Gleason score <or= 6), whereas a complete loss was observed in high-grade cancer specimens (Gleason score > 6). Immunohistochemical analysis further confirmed high levels of S100A2 in benign tissues and a progressive loss with increasing tumor grade. The protein level of S100A4 was significantly higher in all carcinoma cells compared with NHPE and PZ-HPV-7 cells. The mRNA and protein level of S100A4 was significantly higher in high-grade cancer specimens compared with BPH, prostatitis, and low-grade cancer. The high levels of S100A4 observed in cancer tissue correlated with increasing tumor grade. CONCLUSION: Loss of S100A2 and increased expression of S100A4 may be an important event during progression of prostate cancer in humans.
Authors: Mohammad Saleem; Mee-Hyang Kweon; Jeremy James Johnson; Vaqar Mustafa Adhami; Irina Elcheva; Naghma Khan; Bilal Bin Hafeez; Kumar M R Bhat; Sami Sarfaraz; Shannon Reagan-Shaw; Vladimir S Spiegelman; Vijayasaradhi Setaluri; Hasan Mukhtar Journal: Proc Natl Acad Sci U S A Date: 2006-09-21 Impact factor: 11.205
Authors: L Senolt; M Grigorian; E Lukanidin; B Simmen; B A Michel; K Pavelka; R E Gay; S Gay; M Neidhart Journal: Ann Rheum Dis Date: 2006-12 Impact factor: 19.103
Authors: Nuria Mencía; Elisabet Selga; Isabel Rico; M Cristina de Almagro; Xenia Villalobos; Sara Ramirez; Jaume Adan; Jose L Hernández; Véronique Noé; Carlos J Ciudad Journal: BMC Cancer Date: 2010-06-01 Impact factor: 4.430
Authors: Hifzur R Siddique; Vaqar M Adhami; Aijaz Parray; Jeremy J Johnson; Imtiaz A Siddiqui; Mohammad T Shekhani; Imtiyaz Murtaza; Noona Ambartsumian; Badrinath R Konety; Hasan Mukhtar; Mohammad Saleem Journal: Genes Cancer Date: 2013-05